Proteomic analysis of Arabidopsis glutathione S-transferases from benoxacor- and copper-treated seedlings

Aaron P. Smith, Ben P. DeRidder, Woei-Jiun Guo, Erin H. Seeley, Fred E. Regnier, Peter B. Goldsbrough

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

Glutathione S-transferases (GSTs) are involved in many stress responses in plants, for example, participating in the detoxification of xenobiotics and limiting oxidative damage. Studies examining the regulation of this gene family in diverse plant species have focused primarily on RNA expression. A proteomics method was developed to identify GSTs expressed in Arabidopsis seedlings and to determine how the abundance of these proteins changed in response to copper, a promoter of oxidative stress, and benoxacor, a herbicide safener. Eight GSTs were identified in seedlings grown under control conditions, and only one, AtGSTU19, was induced by benoxacor. In contrast, four GSTs, AtGSTF2, AtGSTF6, AtGSTF7, and AtGSTU19, were significantly more abundant in copper-treated seedlings. The different responses to these treatments may reflect the potential for copper to affect many more aspects of plant growth and physiology compared with a herbicide safener. Differences between RNA and protein expression of GSTs indicate that both transcriptional and translational mechanisms are involved in regulation of GSTs under these conditions.

Original languageEnglish
Pages (from-to)26098-26104
Number of pages7
JournalJournal of Biological Chemistry
Volume279
Issue number25
DOIs
Publication statusPublished - 2004 Jun 18

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Glutathione Transferase
Seedlings
Arabidopsis
Proteomics
Copper
Herbicides
Plant Physiological Phenomena
RNA
Detoxification
Oxidative stress
Physiology
Xenobiotics
benoxacor
Proteins
Oxidative Stress
Genes
Growth

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

Smith, Aaron P. ; DeRidder, Ben P. ; Guo, Woei-Jiun ; Seeley, Erin H. ; Regnier, Fred E. ; Goldsbrough, Peter B. / Proteomic analysis of Arabidopsis glutathione S-transferases from benoxacor- and copper-treated seedlings. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 25. pp. 26098-26104.
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Proteomic analysis of Arabidopsis glutathione S-transferases from benoxacor- and copper-treated seedlings. / Smith, Aaron P.; DeRidder, Ben P.; Guo, Woei-Jiun; Seeley, Erin H.; Regnier, Fred E.; Goldsbrough, Peter B.

In: Journal of Biological Chemistry, Vol. 279, No. 25, 18.06.2004, p. 26098-26104.

Research output: Contribution to journalArticle

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