Proteomic analysis of proteins associated with tt-DDE induced toxicity in BEAS-2B cells

Pin Pin Lin; Ming Hui Yang; Pao Chi Liao; Hsin Yi Wu; Louis W. Chang; Hui Ti Tsai; Yu Chang Tyan

doi:10.1016/j.bbrc.2008.09.020

Proteomic analysis of proteins associated with tt-DDE induced toxicity in BEAS-2B cells

Pin Pin Lin, Ming Hui Yang, Pao Chi Liao, Hsin Yi Wu, Louis W. Chang, Hui Ti Tsai, Yu Chang Tyan

Department of Environmental and Occupational Health

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Trans, trans-2,4-decadienal (tt-DDE), a specific type of dienaldehyde, is abundant in heated oils or cooking oil fumes. Ingestion of heated oils and exposure to cooking oil fumes has been suggested to have a great health impact in a variety of organs, including the lungs. Previous studies have demonstrated that acute exposures to high doses of tt-DDE have induced oxidative stress, genotoxicity, and cytotoxicity in human lung cells. The objective in utilizing proteomic techniques of this study was to identify protein biomarkers associated with tt-DDE-induced oxidative stress and cytotoxicity in human bronchial epithelial cells BEAS-2B. Experimental results suggested that DJ-1 and cofilin proteins were protein biomarkers for tt-DDE-induced cytotoxicity and oxidative stress in lung cells. DJ-1 was especially an early biomarker for tt-DDE exposure.

Original language	English
Pages (from-to)	519-524
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	376
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2008.09.020
Publication status	Published - 2008 Nov 21

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2008.09.020

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title = "Proteomic analysis of proteins associated with tt-DDE induced toxicity in BEAS-2B cells",

abstract = "Trans, trans-2,4-decadienal (tt-DDE), a specific type of dienaldehyde, is abundant in heated oils or cooking oil fumes. Ingestion of heated oils and exposure to cooking oil fumes has been suggested to have a great health impact in a variety of organs, including the lungs. Previous studies have demonstrated that acute exposures to high doses of tt-DDE have induced oxidative stress, genotoxicity, and cytotoxicity in human lung cells. The objective in utilizing proteomic techniques of this study was to identify protein biomarkers associated with tt-DDE-induced oxidative stress and cytotoxicity in human bronchial epithelial cells BEAS-2B. Experimental results suggested that DJ-1 and cofilin proteins were protein biomarkers for tt-DDE-induced cytotoxicity and oxidative stress in lung cells. DJ-1 was especially an early biomarker for tt-DDE exposure.",

author = "Lin, {Pin Pin} and Yang, {Ming Hui} and Liao, {Pao Chi} and Wu, {Hsin Yi} and Chang, {Louis W.} and Tsai, {Hui Ti} and Tyan, {Yu Chang}",

note = "Funding Information: The authors thank National Cheng-Kung University Proteomics Research Core Laboratory for the assistance in protein identification. We also thank S. Sheldon, MT (ASCP) of Edmond Medical Center Laboratory (USA) for fruitful discussions. This work was supported by research Grants, DOH94-TD-G-111-010 from the National Research Program for Genomic Medicine and Department of Health, EO-094-PP-09 from Division of Environmental Health and Occupational Medicine, National Health Research Institutes, and NSC96-2321-B-037-006 from the National Science Council, Taiwan, ROC. ",

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doi = "10.1016/j.bbrc.2008.09.020",

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AU - Lin, Pin Pin

AU - Yang, Ming Hui

AU - Liao, Pao Chi

AU - Wu, Hsin Yi

AU - Chang, Louis W.

AU - Tsai, Hui Ti

AU - Tyan, Yu Chang

N1 - Funding Information: The authors thank National Cheng-Kung University Proteomics Research Core Laboratory for the assistance in protein identification. We also thank S. Sheldon, MT (ASCP) of Edmond Medical Center Laboratory (USA) for fruitful discussions. This work was supported by research Grants, DOH94-TD-G-111-010 from the National Research Program for Genomic Medicine and Department of Health, EO-094-PP-09 from Division of Environmental Health and Occupational Medicine, National Health Research Institutes, and NSC96-2321-B-037-006 from the National Science Council, Taiwan, ROC.

PY - 2008/11/21

Y1 - 2008/11/21

N2 - Trans, trans-2,4-decadienal (tt-DDE), a specific type of dienaldehyde, is abundant in heated oils or cooking oil fumes. Ingestion of heated oils and exposure to cooking oil fumes has been suggested to have a great health impact in a variety of organs, including the lungs. Previous studies have demonstrated that acute exposures to high doses of tt-DDE have induced oxidative stress, genotoxicity, and cytotoxicity in human lung cells. The objective in utilizing proteomic techniques of this study was to identify protein biomarkers associated with tt-DDE-induced oxidative stress and cytotoxicity in human bronchial epithelial cells BEAS-2B. Experimental results suggested that DJ-1 and cofilin proteins were protein biomarkers for tt-DDE-induced cytotoxicity and oxidative stress in lung cells. DJ-1 was especially an early biomarker for tt-DDE exposure.

AB - Trans, trans-2,4-decadienal (tt-DDE), a specific type of dienaldehyde, is abundant in heated oils or cooking oil fumes. Ingestion of heated oils and exposure to cooking oil fumes has been suggested to have a great health impact in a variety of organs, including the lungs. Previous studies have demonstrated that acute exposures to high doses of tt-DDE have induced oxidative stress, genotoxicity, and cytotoxicity in human lung cells. The objective in utilizing proteomic techniques of this study was to identify protein biomarkers associated with tt-DDE-induced oxidative stress and cytotoxicity in human bronchial epithelial cells BEAS-2B. Experimental results suggested that DJ-1 and cofilin proteins were protein biomarkers for tt-DDE-induced cytotoxicity and oxidative stress in lung cells. DJ-1 was especially an early biomarker for tt-DDE exposure.

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Proteomic analysis of proteins associated with tt-DDE induced toxicity in BEAS-2B cells

Abstract

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