TY - JOUR
T1 - Proteomic characterization of extracellular vesicles derived from lactic acid bacteria
AU - Lee, Bao Hong
AU - Chen, You Zuo
AU - Shen, Tang Long
AU - Pan, Tzu Ming
AU - Hsu, Wei Hsuan
N1 - Funding Information:
This research work and subsidiary spending were mainly supported by the Ministry of Science and Technology (MOST) in Taiwan under grant nos. MOST 110-2636-B-006-002 (Young Scholar Fellowship Program). This research was also supported in part by Higher Education Sprout Project, Ministry of Education to the Headquarters of University Advancement at National Cheng Kung University (NCKU). The authors gratefully acknowledge the use of EM000800 belonging to the Core Facility Center of National Cheng Kung University. We also thank the technical services provided by the Bioimaging Core Facility of the National Core Facility for Biopharmaceuticals, National Science and Technology Council, Taiwan.
Publisher Copyright:
© 2023 Elsevier Ltd
PY - 2023/11/30
Y1 - 2023/11/30
N2 - Lactobacillus species confer health benefits by their metabolites, secreted molecules, and population numbers. Extracellular vesicles (EVs) are nano-sized particles released from cells and mediate intercellular communications. EVs-encapsulated cargos are a crucial key to decide involved biological function. However, little is known about the composition of EVs, leaving mechanisms by which Lactobacillus-derived EVs affect recipient cells remaining unresolved. This study examined the composition of EV proteins from Lactobacillus species by using liquid chromatography coupled with tandem mass spectrometry, including L. plantarum, L. fermentum, and L. gasseri. The major proteins of EVs are associated with biological processes such as catalytic activity, gluco-neogenesis, cell wall organization, and glycolytic processes. Motif enrichment analysis revealed that EVs from L. plantarum and L. fermentum contained proteins with serine-rich motif. This is the first study to report the composition and comparison of EV proteins from Lactobacillus species, providing important information of EVs in functional food products development.
AB - Lactobacillus species confer health benefits by their metabolites, secreted molecules, and population numbers. Extracellular vesicles (EVs) are nano-sized particles released from cells and mediate intercellular communications. EVs-encapsulated cargos are a crucial key to decide involved biological function. However, little is known about the composition of EVs, leaving mechanisms by which Lactobacillus-derived EVs affect recipient cells remaining unresolved. This study examined the composition of EV proteins from Lactobacillus species by using liquid chromatography coupled with tandem mass spectrometry, including L. plantarum, L. fermentum, and L. gasseri. The major proteins of EVs are associated with biological processes such as catalytic activity, gluco-neogenesis, cell wall organization, and glycolytic processes. Motif enrichment analysis revealed that EVs from L. plantarum and L. fermentum contained proteins with serine-rich motif. This is the first study to report the composition and comparison of EV proteins from Lactobacillus species, providing important information of EVs in functional food products development.
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U2 - 10.1016/j.foodchem.2023.136685
DO - 10.1016/j.foodchem.2023.136685
M3 - Article
C2 - 37356267
AN - SCOPUS:85162986077
SN - 0308-8146
VL - 427
JO - Food Chemistry
JF - Food Chemistry
M1 - 136685
ER -