Proteomic comparison of historic and recently emerged hypervirulent clostridium difficile strains

Jenn Wei Chen, Joy Scaria, Chunhong Mao, Bruno Sobral, Sheng Zhang, Trevor Lawley, Yung Fu Chang

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)


Clostridium difficile in recent years has undergone rapid evolution and has emerged as a serious human pathogen. Proteomic approaches can improve the understanding of the diversity of this important pathogen, especially in comparing the adaptive ability of different C. difficile strains. In this study, TMT labeling and nanoLC-MS/MS driven proteomics were used to investigate the responses of four C. difficile strains to nutrient shift and osmotic shock. We detected 126 and 67 differentially expressed proteins in at least one strain under nutrition shift and osmotic shock, respectively. During nutrient shift, several components of the phosphotransferase system (PTS) were found to be differentially expressed, which indicated that the carbon catabolite repression (CCR) was relieved to allow the expression of enzymes and transporters responsible for the utilization of alternate carbon sources. Some classical osmotic shock associated proteins, such as GroEL, RecA, CspG, and CspF, and other stress proteins such as PurG and SerA were detected during osmotic shock. Furthermore, the recently emerged strains were found to contain a more robust gene network in response to both stress conditions. This work represents the first comparative proteomic analysis of historic and recently emerged hypervirulent C. difficile strains, complementing the previously published proteomics studies utilizing only one reference strain.

Original languageEnglish
Pages (from-to)1151-1161
Number of pages11
JournalJournal of Proteome Research
Issue number3
Publication statusPublished - 2013 Mar

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • General Chemistry


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