Proteomics analysis of plasma for potential biomarkers in the diagnosis of Alzheimer's disease

Pao-Chi Liao, Lung Yu, Chih Chieh Kuo, Chingju Lin, Yu-Min Kuo

Research output: Contribution to journalArticlepeer-review

41 Citations (Scopus)

Abstract

The objective of this study was to search for biological markers associated with Alzheimer's disease (AD). Plasma specimens obtained from ten pathologically diagnosed AD patients and ten non-demented (ND) control subjects were analyzed by a combination of 2-DE and MS. This strategy allowed us to identify six plasma proteins (alpha-1-antitrypsin, vitamin D-binding protein, inter-alpha-trypsin inhibitor family heavy chain-related protein, apolipoprotein J precursor, cAMP-dependent protein kinase catalytic subunit alpha 1, and an orf) whose 2-DE spot densities were different between the AD and ND groups. Due to their involvements in AD amyloid plaque formation, the plasma concentrations of alpha-1-antitrypsin and apolipoprotein J were further validated using either ELISA or Western blot. The results revealed that the plasma levels of alpha-1-antitrypsin in AD were higher than those of controls, confirming the 2-DE findings. However, no difference in total apolipoprotein J concentration was observed between the AD and ND groups. Considering the difference in resolving power to differentially quantitate protein isoforms provided by 2-DE and Western blot, 2-DE analysis combined with MS protein identification offers distinctive advantages when a disease-related protein isoform-specific variance is investigated.

Original languageEnglish
Pages (from-to)506-512
Number of pages7
JournalProteomics - Clinical Applications
Volume1
Issue number5
DOIs
Publication statusPublished - 2007 May 1

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry

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