Thymic hormones have immunomodulatory effects on T cells and hence have been used clinically to restore the immunity of immunodeficient patients as well as to enhance the cellular immunity of cancer patients. Prothymosin α, which is a member of the thymic hormone family, has recently been suggested to act as a nuclear protein participating in the stimulation of cell proliferation. To characterize the biological activities of prothymosin a in vitro, we established NIH3T3 cell transformants that constitutively express higher prothymosin a protein and its mRNA compared with the wild-type counterpart. Cells that overexpressed prothymosin a increased the proliferative activity assayed by the [3H]-thymidine incorporation or by the cell cycle analysis with the fluorescent-activate cell sorter. The results provide direct evidence that prothymosin a plays a role in cell proliferation by shortening the duration of the G1 phase.
All Science Journal Classification (ASJC) codes
- Biochemistry, Genetics and Molecular Biology(all)
- Pharmacology, Toxicology and Pharmaceutics(all)