Pseudoxanthomonas kaohsiungensis, sp. nov., a novel bacterium isolated from oil-polluted site produces extracellular surface activity

Jo-Shu Chang, Cheng Liang Chou, Guang Huey Lin, Shih Yi Sheu, Wen Ming Chen

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

During screening for biosurfactant-producing bacteria, a strain designated J36 T was isolated from oil-polluted site near Kaohsiung city located in southern Taiwan. Cells of this organism were Gram-negative rods motile by means of a single polar flagellum. Strain J36 T grew well in complex media under optimum conditions of 35°C and pH 7. The extracellular products of the strain expressed emulsification activity. During cultivation on olive oil as the sole carbon and energy source, the culture supernatant of strain J36 T reduced surface tension of the medium from 68 to 32.6 dyne/cm. The 16S rRNA gene sequence analysis indicates that strain J36 T is a member of Xanthomonas group within the γ-Proteobacteria. The organism belongs to the genus Pseudoxanthomonas and represents a novel species within this genus according to phylogenetic analysis of 16S rDNA sequences, DNA-DNA similarity data, whole-cell protein analysis, physiological and biochemical characteristics, as well as fatty acid compositions. The predominant cellular fatty acids of strain J36 T were 15:0 iso (about 26%), 17:1 iso ω9c (about 25%), and 15:0 anteiso (about 10%). Its DNA base ratio was 60.1 mol% G+C. We propose to classify strain J36 T (=BCRC 17375 T =LMG 22530 T ) as Pseudoxanthomonas kaohsiungensis sp. nov.

Original languageEnglish
Pages (from-to)137-144
Number of pages8
JournalSystematic and Applied Microbiology
Volume28
Issue number2
DOIs
Publication statusPublished - 2005 Mar 16

Fingerprint

Pseudoxanthomonas
Oils
Fatty Acids
Xanthomonas
Bacteria
DNA
Proteobacteria
oils
bacterium
Surface Tension
Flagella
new species
oil
bacteria
Base Composition
fatty acid
Ribosomal DNA
Taiwan
rRNA Genes
Sequence Analysis

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics
  • Applied Microbiology and Biotechnology

Cite this

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title = "Pseudoxanthomonas kaohsiungensis, sp. nov., a novel bacterium isolated from oil-polluted site produces extracellular surface activity",
abstract = "During screening for biosurfactant-producing bacteria, a strain designated J36 T was isolated from oil-polluted site near Kaohsiung city located in southern Taiwan. Cells of this organism were Gram-negative rods motile by means of a single polar flagellum. Strain J36 T grew well in complex media under optimum conditions of 35°C and pH 7. The extracellular products of the strain expressed emulsification activity. During cultivation on olive oil as the sole carbon and energy source, the culture supernatant of strain J36 T reduced surface tension of the medium from 68 to 32.6 dyne/cm. The 16S rRNA gene sequence analysis indicates that strain J36 T is a member of Xanthomonas group within the γ-Proteobacteria. The organism belongs to the genus Pseudoxanthomonas and represents a novel species within this genus according to phylogenetic analysis of 16S rDNA sequences, DNA-DNA similarity data, whole-cell protein analysis, physiological and biochemical characteristics, as well as fatty acid compositions. The predominant cellular fatty acids of strain J36 T were 15:0 iso (about 26{\%}), 17:1 iso ω9c (about 25{\%}), and 15:0 anteiso (about 10{\%}). Its DNA base ratio was 60.1 mol{\%} G+C. We propose to classify strain J36 T (=BCRC 17375 T =LMG 22530 T ) as Pseudoxanthomonas kaohsiungensis sp. nov.",
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Pseudoxanthomonas kaohsiungensis, sp. nov., a novel bacterium isolated from oil-polluted site produces extracellular surface activity. / Chang, Jo-Shu; Chou, Cheng Liang; Lin, Guang Huey; Sheu, Shih Yi; Chen, Wen Ming.

In: Systematic and Applied Microbiology, Vol. 28, No. 2, 16.03.2005, p. 137-144.

Research output: Contribution to journalArticle

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