Purification and characterization of a protease extracellularly produced by Monascus purpureus CCRC31499 in a shrimp and crab shell powder medium

Tzu Wen Liang, Jane Jean Lin, Yue Horng Yen, Chuan Lu Wang, San Lang Wang

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Monascus purpureus CCRC31499 produced a protease when it was grown in a medium containing shrimp and crab shell powder (SCSP) of marine wastes. An extracellular protease was purified from the culture supernatant to homology. The protease had a molecular weight of 40,000 and a pI of 7.9. The optimal pH, optimum temperature, pH stability, and thermal stability of the protease were pH 7-9, 40°C, pH 5-9, and 40°C, respectively. In addition to protease activity, CCRC31499 also exhibited activity of enhancing vegetable growth in culture supernatant. This is also the first report of isolation of a protease from Monascus species.

Original languageEnglish
Pages (from-to)74-80
Number of pages7
JournalEnzyme and Microbial Technology
Volume38
Issue number1-2
DOIs
Publication statusPublished - 2006 Jan 3

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

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