Purification and identification of transglutaminase from mouse coagulating gland and its cross-linking activity among seminal vesicle secretion proteins

Huan Chin Tseng, Han Jia Lin, P. S. Sudhakar Gandhi, Chia-Yih Wang, Yee Hsiung Chen

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A 75-kDa protein secreted from mouse coagulating gland was purified to homogeneity by a series of isolation steps including ion exchange chromatography on a DEAE-Sephacel column and ion exchange high-performance liquid chromatography on a sulfopropyl column. It was identified to be Type IV transglutaminase (TG4), based on the establishment of N-terminal sequences by automated Edman degradation together with partial sequences by MS analysis. Its cross-linking activity was tested on the reduced sample of mouse seminal secretion which contained seven major monomer proteins tentatively designated as SVS I-VII. The enzyme was able to cross-link any of SVS I-III but failed to cross-link the other SVS proteins with a Mr value less than 14 kDa. SVS I and SVS III showed comparable substrate activity, but were much weaker than SVS II during the TG4 catalysis.

Original languageEnglish
Pages (from-to)198-202
Number of pages5
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume876
Issue number2
DOIs
Publication statusPublished - 2008 Dec 15

Fingerprint

Seminal Vesicle Secretory Proteins
Transglutaminases
Purification
Ion exchange
Proteins
Ion Exchange
Ion Exchange Chromatography
High performance liquid chromatography
Chromatography
Catalysis
Sequence Analysis
Monomers
High Pressure Liquid Chromatography
Degradation
Substrates
Enzymes

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

Cite this

@article{6b618ad317a34d70ad3f627c2a68d7bd,
title = "Purification and identification of transglutaminase from mouse coagulating gland and its cross-linking activity among seminal vesicle secretion proteins",
abstract = "A 75-kDa protein secreted from mouse coagulating gland was purified to homogeneity by a series of isolation steps including ion exchange chromatography on a DEAE-Sephacel column and ion exchange high-performance liquid chromatography on a sulfopropyl column. It was identified to be Type IV transglutaminase (TG4), based on the establishment of N-terminal sequences by automated Edman degradation together with partial sequences by MS analysis. Its cross-linking activity was tested on the reduced sample of mouse seminal secretion which contained seven major monomer proteins tentatively designated as SVS I-VII. The enzyme was able to cross-link any of SVS I-III but failed to cross-link the other SVS proteins with a Mr value less than 14 kDa. SVS I and SVS III showed comparable substrate activity, but were much weaker than SVS II during the TG4 catalysis.",
author = "Tseng, {Huan Chin} and Lin, {Han Jia} and {Sudhakar Gandhi}, {P. S.} and Chia-Yih Wang and Chen, {Yee Hsiung}",
year = "2008",
month = "12",
day = "15",
doi = "10.1016/j.jchromb.2008.10.041",
language = "English",
volume = "876",
pages = "198--202",
journal = "Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences",
issn = "1570-0232",
publisher = "Elsevier",
number = "2",

}

Purification and identification of transglutaminase from mouse coagulating gland and its cross-linking activity among seminal vesicle secretion proteins. / Tseng, Huan Chin; Lin, Han Jia; Sudhakar Gandhi, P. S.; Wang, Chia-Yih; Chen, Yee Hsiung.

In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 876, No. 2, 15.12.2008, p. 198-202.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Purification and identification of transglutaminase from mouse coagulating gland and its cross-linking activity among seminal vesicle secretion proteins

AU - Tseng, Huan Chin

AU - Lin, Han Jia

AU - Sudhakar Gandhi, P. S.

AU - Wang, Chia-Yih

AU - Chen, Yee Hsiung

PY - 2008/12/15

Y1 - 2008/12/15

N2 - A 75-kDa protein secreted from mouse coagulating gland was purified to homogeneity by a series of isolation steps including ion exchange chromatography on a DEAE-Sephacel column and ion exchange high-performance liquid chromatography on a sulfopropyl column. It was identified to be Type IV transglutaminase (TG4), based on the establishment of N-terminal sequences by automated Edman degradation together with partial sequences by MS analysis. Its cross-linking activity was tested on the reduced sample of mouse seminal secretion which contained seven major monomer proteins tentatively designated as SVS I-VII. The enzyme was able to cross-link any of SVS I-III but failed to cross-link the other SVS proteins with a Mr value less than 14 kDa. SVS I and SVS III showed comparable substrate activity, but were much weaker than SVS II during the TG4 catalysis.

AB - A 75-kDa protein secreted from mouse coagulating gland was purified to homogeneity by a series of isolation steps including ion exchange chromatography on a DEAE-Sephacel column and ion exchange high-performance liquid chromatography on a sulfopropyl column. It was identified to be Type IV transglutaminase (TG4), based on the establishment of N-terminal sequences by automated Edman degradation together with partial sequences by MS analysis. Its cross-linking activity was tested on the reduced sample of mouse seminal secretion which contained seven major monomer proteins tentatively designated as SVS I-VII. The enzyme was able to cross-link any of SVS I-III but failed to cross-link the other SVS proteins with a Mr value less than 14 kDa. SVS I and SVS III showed comparable substrate activity, but were much weaker than SVS II during the TG4 catalysis.

UR - http://www.scopus.com/inward/record.url?scp=56549088408&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=56549088408&partnerID=8YFLogxK

U2 - 10.1016/j.jchromb.2008.10.041

DO - 10.1016/j.jchromb.2008.10.041

M3 - Article

C2 - 19027372

AN - SCOPUS:56549088408

VL - 876

SP - 198

EP - 202

JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

SN - 1570-0232

IS - 2

ER -