Qualification and Verification of Serological Biomarker Candidates for Lung Adenocarcinoma by Targeted Mass Spectrometry

Hsin Yi Wu, Yih Gang Goan, Ying Hua Chang, Yi Fang Yang, Hsiao Jen Chang, Pin Nan Cheng, Chih Chieh Wu, Victor G. Zgoda, Yu Ju Chen, Pao Chi Liao

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Lung cancer is the leading cause of cancer mortality worldwide. Although many biomarkers have been identified for lung cancer, their low specificity and sensitivity present an urgent need for the identification of more candidate biomarkers. In this study, we conducted MRM-based targeted analysis to evaluate the potential utility of a list of candidate proteins for lung cancer diagnosis. A total of 1249 transitions of 420 peptides representing 102 candidate proteins from our previous study and the literature were first screened by MRM analysis in pooled plasma samples, resulting in 78 proteins remaining in the list. Relative quantification of these 78 proteins was further performed in 60 individual plasma samples from lung adenocarcinoma patients in stages I-III and matched healthy control subjects. Ultimately, nine proteins were found to be able to distinguish patients from controls. Further combinations of five, three, and two candidate marker proteins improved the sensitivity to discriminate patients from controls and resulted in a merged AUC value of nearly 1.00 in stages I-III patients versus controls. Our results highlighted several possible markers for lung adenocarcinoma, and the proposed protein panels require further validation in a larger cohort to evaluate their potential use in clinical applications or development of therapeutics.

Original languageEnglish
Pages (from-to)3039-3050
Number of pages12
JournalJournal of Proteome Research
Volume14
Issue number8
DOIs
Publication statusPublished - 2015 Aug 7

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Biomarkers
Mass spectrometry
Mass Spectrometry
Proteins
Lung Neoplasms
Plasmas
Adenocarcinoma of lung
Area Under Curve
Healthy Volunteers
Sensitivity and Specificity
Peptides
Mortality
Neoplasms

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Chemistry(all)

Cite this

Wu, Hsin Yi ; Goan, Yih Gang ; Chang, Ying Hua ; Yang, Yi Fang ; Chang, Hsiao Jen ; Cheng, Pin Nan ; Wu, Chih Chieh ; Zgoda, Victor G. ; Chen, Yu Ju ; Liao, Pao Chi. / Qualification and Verification of Serological Biomarker Candidates for Lung Adenocarcinoma by Targeted Mass Spectrometry. In: Journal of Proteome Research. 2015 ; Vol. 14, No. 8. pp. 3039-3050.
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Qualification and Verification of Serological Biomarker Candidates for Lung Adenocarcinoma by Targeted Mass Spectrometry. / Wu, Hsin Yi; Goan, Yih Gang; Chang, Ying Hua; Yang, Yi Fang; Chang, Hsiao Jen; Cheng, Pin Nan; Wu, Chih Chieh; Zgoda, Victor G.; Chen, Yu Ju; Liao, Pao Chi.

In: Journal of Proteome Research, Vol. 14, No. 8, 07.08.2015, p. 3039-3050.

Research output: Contribution to journalArticle

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AU - Chang, Hsiao Jen

AU - Cheng, Pin Nan

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AU - Liao, Pao Chi

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