Abstract
The determination of cell confluency and subculture timing for cell culture consistency is crucial in the field of cell-based research, but there is no universal standard concerning optimal confluence. In this study, gold nanodot arrays on glass substrates were used as culture substrates, and their spectral shifts of localized surface plasmon resonance (LSPR) were employed to monitor cell growth and quantify cell confluency. Experiments including cell counting, metabolic activity, focal adhesion, and cell cycle were also performed to confirm the cell growth monitoring accuracy of the LSPR signals. The LSPR signal exhibited the same trends like the increase of cell numbers and cell metabolic activity and reached the maximum as the cell growth achieved confluency, suggesting its great capability as an effective indicator to predict suitable subculture timing. The proposed sensing approach is a noninterventional, nondestructive, real-time, and useful tool to help biologists quantify the optimal subculture timing, achieve cell culture consistency, and obtain reproducible experimental results efficiently.
| Original language | English |
|---|---|
| Pages (from-to) | 1816-1824 |
| Number of pages | 9 |
| Journal | ACS Sensors |
| Volume | 4 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 2019 Jul 26 |
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All Science Journal Classification (ASJC) codes
- Bioengineering
- Instrumentation
- Process Chemistry and Technology
- Fluid Flow and Transfer Processes
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Quantifying cell confluency by plasmonic nanodot arrays to achieve cultivating consistency. / Chang, Wen Huei; Yang, Zi Yi; Chong, Tak Wang; Liu, Ya Yu; Pan, Hung Wei; Lin, Chun-Hung.
In: ACS Sensors, Vol. 4, No. 7, 26.07.2019, p. 1816-1824.Research output: Contribution to journal › Article
TY - JOUR
T1 - Quantifying cell confluency by plasmonic nanodot arrays to achieve cultivating consistency
AU - Chang, Wen Huei
AU - Yang, Zi Yi
AU - Chong, Tak Wang
AU - Liu, Ya Yu
AU - Pan, Hung Wei
AU - Lin, Chun-Hung
PY - 2019/7/26
Y1 - 2019/7/26
N2 - The determination of cell confluency and subculture timing for cell culture consistency is crucial in the field of cell-based research, but there is no universal standard concerning optimal confluence. In this study, gold nanodot arrays on glass substrates were used as culture substrates, and their spectral shifts of localized surface plasmon resonance (LSPR) were employed to monitor cell growth and quantify cell confluency. Experiments including cell counting, metabolic activity, focal adhesion, and cell cycle were also performed to confirm the cell growth monitoring accuracy of the LSPR signals. The LSPR signal exhibited the same trends like the increase of cell numbers and cell metabolic activity and reached the maximum as the cell growth achieved confluency, suggesting its great capability as an effective indicator to predict suitable subculture timing. The proposed sensing approach is a noninterventional, nondestructive, real-time, and useful tool to help biologists quantify the optimal subculture timing, achieve cell culture consistency, and obtain reproducible experimental results efficiently.
AB - The determination of cell confluency and subculture timing for cell culture consistency is crucial in the field of cell-based research, but there is no universal standard concerning optimal confluence. In this study, gold nanodot arrays on glass substrates were used as culture substrates, and their spectral shifts of localized surface plasmon resonance (LSPR) were employed to monitor cell growth and quantify cell confluency. Experiments including cell counting, metabolic activity, focal adhesion, and cell cycle were also performed to confirm the cell growth monitoring accuracy of the LSPR signals. The LSPR signal exhibited the same trends like the increase of cell numbers and cell metabolic activity and reached the maximum as the cell growth achieved confluency, suggesting its great capability as an effective indicator to predict suitable subculture timing. The proposed sensing approach is a noninterventional, nondestructive, real-time, and useful tool to help biologists quantify the optimal subculture timing, achieve cell culture consistency, and obtain reproducible experimental results efficiently.
UR - http://www.scopus.com/inward/record.url?scp=85070558634&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85070558634&partnerID=8YFLogxK
U2 - 10.1021/acssensors.9b00524
DO - 10.1021/acssensors.9b00524
M3 - Article
C2 - 31251034
AN - SCOPUS:85070558634
VL - 4
SP - 1816
EP - 1824
JO - ACS Sensors
JF - ACS Sensors
SN - 2379-3694
IS - 7
ER -