Quantifying cell confluency by plasmonic nanodot arrays to achieve cultivating consistency

Wen Huei Chang, Zi Yi Yang, Tak Wang Chong, Ya Yu Liu, Hung Wei Pan, Chun-Hung Lin

Research output: Contribution to journalArticle

Abstract

The determination of cell confluency and subculture timing for cell culture consistency is crucial in the field of cell-based research, but there is no universal standard concerning optimal confluence. In this study, gold nanodot arrays on glass substrates were used as culture substrates, and their spectral shifts of localized surface plasmon resonance (LSPR) were employed to monitor cell growth and quantify cell confluency. Experiments including cell counting, metabolic activity, focal adhesion, and cell cycle were also performed to confirm the cell growth monitoring accuracy of the LSPR signals. The LSPR signal exhibited the same trends like the increase of cell numbers and cell metabolic activity and reached the maximum as the cell growth achieved confluency, suggesting its great capability as an effective indicator to predict suitable subculture timing. The proposed sensing approach is a noninterventional, nondestructive, real-time, and useful tool to help biologists quantify the optimal subculture timing, achieve cell culture consistency, and obtain reproducible experimental results efficiently.

Original languageEnglish
Pages (from-to)1816-1824
Number of pages9
JournalACS Sensors
Volume4
Issue number7
DOIs
Publication statusPublished - 2019 Jul 26

Fingerprint

Cell growth
Surface plasmon resonance
Cell culture
cells
surface plasmon resonance
time measurement
Substrates
Gold
Adhesion
Cells
Glass
Monitoring
counting
adhesion
Experiments
gold
trends
cycles
glass
shift

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Instrumentation
  • Process Chemistry and Technology
  • Fluid Flow and Transfer Processes

Cite this

Chang, Wen Huei ; Yang, Zi Yi ; Chong, Tak Wang ; Liu, Ya Yu ; Pan, Hung Wei ; Lin, Chun-Hung. / Quantifying cell confluency by plasmonic nanodot arrays to achieve cultivating consistency. In: ACS Sensors. 2019 ; Vol. 4, No. 7. pp. 1816-1824.
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Quantifying cell confluency by plasmonic nanodot arrays to achieve cultivating consistency. / Chang, Wen Huei; Yang, Zi Yi; Chong, Tak Wang; Liu, Ya Yu; Pan, Hung Wei; Lin, Chun-Hung.

In: ACS Sensors, Vol. 4, No. 7, 26.07.2019, p. 1816-1824.

Research output: Contribution to journalArticle

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