Radiolabeled polymerase chain reaction assay for detection of ras oncogene point mutations in tumors

Yoichi Aoki, J. C. Lee, Shiv Pillai, Kurt J. Isselbacher, Anil K. Rustgi

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

The human ras gene plays a fundamental role in the transduction of extracellular signals to the nucleus, thereby regulating cell growth and differentiation. Point mutations in the ras gene convert it into a transforming oncogene that has been found in many solid and hematologic malignancies. We describe a rapid and sensitive assay based on a radiolabeled polymerase chain reaction followed by restriction enzyme digestion that we have adapted for differentiating between the wild-type and mutant ras genes. This assay should prove useful in the analysis of ras gene point mutations in clinical tumor specimens in which ras oncogene activation is an early event in carcinogenesis.

Original languageEnglish
Pages (from-to)705-709
Number of pages5
JournalClinical Chemistry
Volume40
Issue number5
Publication statusPublished - 1994 May 20

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry
  • Biochemistry, medical

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