Raman descrimination of helicobacter pylori fingerprint in dielectrophoresis chip

Chi Chang Lin, Ying Mei Yang, Hsien Chang Chang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A rapid trapping and detection method for clinical bacteria (Helicobacter pylori, H. pylori) analysis is designed and reported in this study. Different strain of H. pylori can be trapped in 3 dimension dielectrophoresis chip (DEP) under the condition of 20 mM PBS, voltage at 20 Vp-p and frequency at 300 kHz. H. pylori #238(Wild type) presents more and stronger functional group stretching and vibration in Raman spectra than that of H. pylori #603(gene knockout). In clinical specimen analysis, significant difference between Duodenal Ulcer (DU, n=6) and Gastric Cancer (GC, n=4) are observed and showed the 70% and 80% of correction rate with clinical analysis results by PCA and HCA analysis. The DEP chip with rapid identification function is successful achieved and the fingerprint of H. pylori can be in situ detected and obtained in 3 min.

Original languageEnglish
Title of host publication4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009
Pages793-796
Number of pages4
DOIs
Publication statusPublished - 2009 Oct 12
Event4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009 - Shenzhen, China
Duration: 2009 Jan 52009 Jan 8

Publication series

Name4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009

Other

Other4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009
CountryChina
CityShenzhen
Period09-01-0509-01-08

All Science Journal Classification (ASJC) codes

  • Electrical and Electronic Engineering

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  • Cite this

    Lin, C. C., Yang, Y. M., & Chang, H. C. (2009). Raman descrimination of helicobacter pylori fingerprint in dielectrophoresis chip. In 4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009 (pp. 793-796). [5068697] (4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009). https://doi.org/10.1109/NEMS.2009.5068697