A rapid trapping and detection method for clinical bacteria (Helicobacter pylori, H. pylori) analysis is designed and reported in this study. Different strain of H. pylori can be trapped in 3 dimension dielectrophoresis chip (DEP) under the condition of 20 mM PBS, voltage at 20 Vp-p and frequency at 300 kHz. H. pylori #238(Wild type) presents more and stronger functional group stretching and vibration in Raman spectra than that of H. pylori #603(gene knockout). In clinical specimen analysis, significant difference between Duodenal Ulcer (DU, n=6) and Gastric Cancer (GC, n=4) are observed and showed the 70% and 80% of correction rate with clinical analysis results by PCA and HCA analysis. The DEP chip with rapid identification function is successful achieved and the fingerprint of H. pylori can be in situ detected and obtained in 3 min.