TY - GEN
T1 - Raman descrimination of helicobacter pylori fingerprint in dielectrophoresis chip
AU - Lin, Chi Chang
AU - Yang, Ying Mei
AU - Chang, Hsien Chang
PY - 2009
Y1 - 2009
N2 - A rapid trapping and detection method for clinical bacteria (Helicobacter pylori, H. pylori) analysis is designed and reported in this study. Different strain of H. pylori can be trapped in 3 dimension dielectrophoresis chip (DEP) under the condition of 20 mM PBS, voltage at 20 Vp-p and frequency at 300 kHz. H. pylori #238(Wild type) presents more and stronger functional group stretching and vibration in Raman spectra than that of H. pylori #603(gene knockout). In clinical specimen analysis, significant difference between Duodenal Ulcer (DU, n=6) and Gastric Cancer (GC, n=4) are observed and showed the 70% and 80% of correction rate with clinical analysis results by PCA and HCA analysis. The DEP chip with rapid identification function is successful achieved and the fingerprint of H. pylori can be in situ detected and obtained in 3 min.
AB - A rapid trapping and detection method for clinical bacteria (Helicobacter pylori, H. pylori) analysis is designed and reported in this study. Different strain of H. pylori can be trapped in 3 dimension dielectrophoresis chip (DEP) under the condition of 20 mM PBS, voltage at 20 Vp-p and frequency at 300 kHz. H. pylori #238(Wild type) presents more and stronger functional group stretching and vibration in Raman spectra than that of H. pylori #603(gene knockout). In clinical specimen analysis, significant difference between Duodenal Ulcer (DU, n=6) and Gastric Cancer (GC, n=4) are observed and showed the 70% and 80% of correction rate with clinical analysis results by PCA and HCA analysis. The DEP chip with rapid identification function is successful achieved and the fingerprint of H. pylori can be in situ detected and obtained in 3 min.
UR - http://www.scopus.com/inward/record.url?scp=70349687742&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=70349687742&partnerID=8YFLogxK
U2 - 10.1109/NEMS.2009.5068697
DO - 10.1109/NEMS.2009.5068697
M3 - Conference contribution
AN - SCOPUS:70349687742
SN - 9781424446308
T3 - 4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009
SP - 793
EP - 796
BT - 4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009
T2 - 4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009
Y2 - 5 January 2009 through 8 January 2009
ER -
