Raman descrimination of helicobacter pylori fingerprint in dielectrophoresis chip

Chi Chang Lin; Ying Mei Yang; Hsien Chang Chang

doi:10.1109/NEMS.2009.5068697

Raman descrimination of helicobacter pylori fingerprint in dielectrophoresis chip

Chi Chang Lin
, Ying Mei Yang
, Hsien Chang Chang

Department of Biomedical Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Citation (Scopus)

Abstract

A rapid trapping and detection method for clinical bacteria (Helicobacter pylori, H. pylori) analysis is designed and reported in this study. Different strain of H. pylori can be trapped in 3 dimension dielectrophoresis chip (DEP) under the condition of 20 mM PBS, voltage at 20 Vp-p and frequency at 300 kHz. H. pylori #238(Wild type) presents more and stronger functional group stretching and vibration in Raman spectra than that of H. pylori #603(gene knockout). In clinical specimen analysis, significant difference between Duodenal Ulcer (DU, n=6) and Gastric Cancer (GC, n=4) are observed and showed the 70% and 80% of correction rate with clinical analysis results by PCA and HCA analysis. The DEP chip with rapid identification function is successful achieved and the fingerprint of H. pylori can be in situ detected and obtained in 3 min.

Original language	English
Title of host publication	4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009
Pages	793-796
Number of pages	4
DOIs	https://doi.org/10.1109/NEMS.2009.5068697
Publication status	Published - 2009
Event	4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009 - Shenzhen, China Duration: 2009 Jan 5 → 2009 Jan 8

Publication series

Name	4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009

Other

Other	4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009
Country/Territory	China
City	Shenzhen
Period	09-01-05 → 09-01-08

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

Electrical and Electronic Engineering

Access to Document

10.1109/NEMS.2009.5068697

Cite this

Lin, C. C., Yang, Y. M., & Chang, H. C. (2009). Raman descrimination of helicobacter pylori fingerprint in dielectrophoresis chip. In 4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009 (pp. 793-796). Article 5068697 (4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009). https://doi.org/10.1109/NEMS.2009.5068697

@inproceedings{96ef65173da74bc787a25667a5e0596b,

title = "Raman descrimination of helicobacter pylori fingerprint in dielectrophoresis chip",

abstract = "A rapid trapping and detection method for clinical bacteria (Helicobacter pylori, H. pylori) analysis is designed and reported in this study. Different strain of H. pylori can be trapped in 3 dimension dielectrophoresis chip (DEP) under the condition of 20 mM PBS, voltage at 20 Vp-p and frequency at 300 kHz. H. pylori \#238(Wild type) presents more and stronger functional group stretching and vibration in Raman spectra than that of H. pylori \#603(gene knockout). In clinical specimen analysis, significant difference between Duodenal Ulcer (DU, n=6) and Gastric Cancer (GC, n=4) are observed and showed the 70\% and 80\% of correction rate with clinical analysis results by PCA and HCA analysis. The DEP chip with rapid identification function is successful achieved and the fingerprint of H. pylori can be in situ detected and obtained in 3 min.",

author = "Lin, \{Chi Chang\} and Yang, \{Ying Mei\} and Chang, \{Hsien Chang\}",

year = "2009",

doi = "10.1109/NEMS.2009.5068697",

language = "English",

isbn = "9781424446308",

series = "4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009",

pages = "793--796",

booktitle = "4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009",

note = "4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009 ; Conference date: 05-01-2009 Through 08-01-2009",

}

Lin, CC, Yang, YM & Chang, HC 2009, Raman descrimination of helicobacter pylori fingerprint in dielectrophoresis chip. in 4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009., 5068697, 4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009, pp. 793-796, 4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009, Shenzhen, China, 09-01-05. https://doi.org/10.1109/NEMS.2009.5068697

Raman descrimination of helicobacter pylori fingerprint in dielectrophoresis chip. / Lin, Chi Chang; Yang, Ying Mei; Chang, Hsien Chang.
4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009. 2009. p. 793-796 5068697 (4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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AU - Lin, Chi Chang

AU - Yang, Ying Mei

AU - Chang, Hsien Chang

PY - 2009

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N2 - A rapid trapping and detection method for clinical bacteria (Helicobacter pylori, H. pylori) analysis is designed and reported in this study. Different strain of H. pylori can be trapped in 3 dimension dielectrophoresis chip (DEP) under the condition of 20 mM PBS, voltage at 20 Vp-p and frequency at 300 kHz. H. pylori #238(Wild type) presents more and stronger functional group stretching and vibration in Raman spectra than that of H. pylori #603(gene knockout). In clinical specimen analysis, significant difference between Duodenal Ulcer (DU, n=6) and Gastric Cancer (GC, n=4) are observed and showed the 70% and 80% of correction rate with clinical analysis results by PCA and HCA analysis. The DEP chip with rapid identification function is successful achieved and the fingerprint of H. pylori can be in situ detected and obtained in 3 min.

AB - A rapid trapping and detection method for clinical bacteria (Helicobacter pylori, H. pylori) analysis is designed and reported in this study. Different strain of H. pylori can be trapped in 3 dimension dielectrophoresis chip (DEP) under the condition of 20 mM PBS, voltage at 20 Vp-p and frequency at 300 kHz. H. pylori #238(Wild type) presents more and stronger functional group stretching and vibration in Raman spectra than that of H. pylori #603(gene knockout). In clinical specimen analysis, significant difference between Duodenal Ulcer (DU, n=6) and Gastric Cancer (GC, n=4) are observed and showed the 70% and 80% of correction rate with clinical analysis results by PCA and HCA analysis. The DEP chip with rapid identification function is successful achieved and the fingerprint of H. pylori can be in situ detected and obtained in 3 min.

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M3 - Conference contribution

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T3 - 4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009

SP - 793

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T2 - 4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009

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ER -

Raman descrimination of helicobacter pylori fingerprint in dielectrophoresis chip

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