Raman mapping glucose metabolism during adipogenesis from human mesenchymal stem cells

S. S. Gomathy; C. Stylianou; I. Y. Phang; S. Cool; V. Nurcombe; F. Ample; M. J. Lear; S. Gorelik; J. Hobley

doi:10.1109/PGC.2010.5705978

Raman mapping glucose metabolism during adipogenesis from human mesenchymal stem cells

S. S. Gomathy, C. Stylianou, I. Y. Phang, S. Cool, V. Nurcombe, F. Ample, M. J. Lear, S. Gorelik, J. Hobley

Department of Biomedical Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

5 Citations (Scopus)

Abstract

Raman mapping was used to determine the distribution of fats inside of adipocytes and human mesenchymal stem cells during adipogenesis. This proved to be a successful way of mapping the lipid distribution within the cells because of the strong C-H stretching bands of the fats inside the lipid droplets. Furthermore using deuterated glucose in the cell culture medium it was possible to distinguish any fats in droplets or in the cytoplasm that were formed before and after adipogenesis was initiated, because those formed after displayed both C-H and C-D stretching bands. The distributions of the initial metabolites of glucose and the resulting fats in the lipid drops could also be mapped in this way.

Original language	English
Title of host publication	2010 Photonics Global Conference, PGC 2010
DOIs	https://doi.org/10.1109/PGC.2010.5705978
Publication status	Published - 2010
Event	2010 Photonics Global Conference, PGC 2010 - Orchard, Singapore Duration: 2010 Dec 14 → 2010 Dec 16

Publication series

Name	2010 Photonics Global Conference, PGC 2010

Conference

Conference	2010 Photonics Global Conference, PGC 2010
Country/Territory	Singapore
City	Orchard
Period	10-12-14 → 10-12-16

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Mathematical Physics
Atomic and Molecular Physics, and Optics

Access to Document

10.1109/PGC.2010.5705978

Cite this

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title = "Raman mapping glucose metabolism during adipogenesis from human mesenchymal stem cells",

abstract = "Raman mapping was used to determine the distribution of fats inside of adipocytes and human mesenchymal stem cells during adipogenesis. This proved to be a successful way of mapping the lipid distribution within the cells because of the strong C-H stretching bands of the fats inside the lipid droplets. Furthermore using deuterated glucose in the cell culture medium it was possible to distinguish any fats in droplets or in the cytoplasm that were formed before and after adipogenesis was initiated, because those formed after displayed both C-H and C-D stretching bands. The distributions of the initial metabolites of glucose and the resulting fats in the lipid drops could also be mapped in this way.",

author = "Gomathy, {S. S.} and C. Stylianou and Phang, {I. Y.} and S. Cool and V. Nurcombe and F. Ample and Lear, {M. J.} and S. Gorelik and J. Hobley",

year = "2010",

doi = "10.1109/PGC.2010.5705978",

language = "English",

isbn = "9781424498826",

series = "2010 Photonics Global Conference, PGC 2010",

booktitle = "2010 Photonics Global Conference, PGC 2010",

}

Gomathy, SS, Stylianou, C, Phang, IY, Cool, S, Nurcombe, V, Ample, F, Lear, MJ, Gorelik, S & Hobley, J 2010, Raman mapping glucose metabolism during adipogenesis from human mesenchymal stem cells. in 2010 Photonics Global Conference, PGC 2010., 5705978, 2010 Photonics Global Conference, PGC 2010, 2010 Photonics Global Conference, PGC 2010, Orchard, Singapore, 10-12-14. https://doi.org/10.1109/PGC.2010.5705978

TY - GEN

T1 - Raman mapping glucose metabolism during adipogenesis from human mesenchymal stem cells

AU - Gomathy, S. S.

AU - Stylianou, C.

AU - Phang, I. Y.

AU - Cool, S.

AU - Nurcombe, V.

AU - Ample, F.

AU - Lear, M. J.

AU - Gorelik, S.

AU - Hobley, J.

PY - 2010

Y1 - 2010

N2 - Raman mapping was used to determine the distribution of fats inside of adipocytes and human mesenchymal stem cells during adipogenesis. This proved to be a successful way of mapping the lipid distribution within the cells because of the strong C-H stretching bands of the fats inside the lipid droplets. Furthermore using deuterated glucose in the cell culture medium it was possible to distinguish any fats in droplets or in the cytoplasm that were formed before and after adipogenesis was initiated, because those formed after displayed both C-H and C-D stretching bands. The distributions of the initial metabolites of glucose and the resulting fats in the lipid drops could also be mapped in this way.

AB - Raman mapping was used to determine the distribution of fats inside of adipocytes and human mesenchymal stem cells during adipogenesis. This proved to be a successful way of mapping the lipid distribution within the cells because of the strong C-H stretching bands of the fats inside the lipid droplets. Furthermore using deuterated glucose in the cell culture medium it was possible to distinguish any fats in droplets or in the cytoplasm that were formed before and after adipogenesis was initiated, because those formed after displayed both C-H and C-D stretching bands. The distributions of the initial metabolites of glucose and the resulting fats in the lipid drops could also be mapped in this way.

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U2 - 10.1109/PGC.2010.5705978

DO - 10.1109/PGC.2010.5705978

M3 - Conference contribution

AN - SCOPUS:79952338628

SN - 9781424498826

T3 - 2010 Photonics Global Conference, PGC 2010

BT - 2010 Photonics Global Conference, PGC 2010

T2 - 2010 Photonics Global Conference, PGC 2010

Y2 - 14 December 2010 through 16 December 2010

ER -

Raman mapping glucose metabolism during adipogenesis from human mesenchymal stem cells

Abstract

Publication series

Conference

All Science Journal Classification (ASJC) codes

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