Rapid and dynamic signal enhancement of bead-based bioassays using a multi-functional optoelectric device

K. C. Wang, F. T. Li, Dar-Bin Shieh, K. C. Kim, Han-Sheng Chuang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Colloidal particles are broadly used in biochemical applications. Considerable research works have proven particles to be useful carriers in many bead-based bioassays. The increasing use of colloidal systems raises demands for a versatile tool capable of performing multiple functions. To this end, this paper presents a simple optoelectrokinetic method for manipulating particles by simultaneously inducing electrohydrodnamic flow and electrokinetic motions. With carefully tuning the balance between several forces acting on particles, manipulation capabilities including aggregation, translation, sorting, and patterning were achieved. The experimental results showed that the technique is subjected to frequency, electric voltage, laser intensity, and particle size. For a proof of concept, the technique was used to enhance the weak signal of a dilute biotin solution by concentrating streptavidin coated particles. Compared with a ELISA reader, the minimal readout was 100 fold improvement. The demonstration provides a good insight to the future biochemical applications.

Original languageEnglish
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PublisherChemical and Biological Microsystems Society
Pages1554-1556
Number of pages3
ISBN (Electronic)9780979806476
Publication statusPublished - 2014 Jan 1
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
Duration: 2014 Oct 262014 Oct 30

Publication series

Name18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

Other

Other18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
CountryUnited States
CitySan Antonio
Period14-10-2614-10-30

Fingerprint

Bioassay
Sorting
Demonstrations
Agglomeration
Tuning
Particle size
Lasers
Electric potential
Biotin

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

Cite this

Wang, K. C., Li, F. T., Shieh, D-B., Kim, K. C., & Chuang, H-S. (2014). Rapid and dynamic signal enhancement of bead-based bioassays using a multi-functional optoelectric device. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 (pp. 1554-1556). (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014). Chemical and Biological Microsystems Society.
Wang, K. C. ; Li, F. T. ; Shieh, Dar-Bin ; Kim, K. C. ; Chuang, Han-Sheng. / Rapid and dynamic signal enhancement of bead-based bioassays using a multi-functional optoelectric device. 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. pp. 1554-1556 (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014).
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Wang, KC, Li, FT, Shieh, D-B, Kim, KC & Chuang, H-S 2014, Rapid and dynamic signal enhancement of bead-based bioassays using a multi-functional optoelectric device. in 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, Chemical and Biological Microsystems Society, pp. 1554-1556, 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, San Antonio, United States, 14-10-26.

Rapid and dynamic signal enhancement of bead-based bioassays using a multi-functional optoelectric device. / Wang, K. C.; Li, F. T.; Shieh, Dar-Bin; Kim, K. C.; Chuang, Han-Sheng.

18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. p. 1554-1556 (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Wang KC, Li FT, Shieh D-B, Kim KC, Chuang H-S. Rapid and dynamic signal enhancement of bead-based bioassays using a multi-functional optoelectric device. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society. 2014. p. 1554-1556. (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014).