Abstract
Colloidal particles are broadly used in biochemical applications. Considerable research works have proven particles to be useful carriers in many bead-based bioassays. The increasing use of colloidal systems raises demands for a versatile tool capable of performing multiple functions. To this end, this paper presents a simple optoelectrokinetic method for manipulating particles by simultaneously inducing electrohydrodnamic flow and electrokinetic motions. With carefully tuning the balance between several forces acting on particles, manipulation capabilities including aggregation, translation, sorting, and patterning were achieved. The experimental results showed that the technique is subjected to frequency, electric voltage, laser intensity, and particle size. For a proof of concept, the technique was used to enhance the weak signal of a dilute biotin solution by concentrating streptavidin coated particles. Compared with a ELISA reader, the minimal readout was 100 fold improvement. The demonstration provides a good insight to the future biochemical applications.
| Original language | English |
|---|---|
| Title of host publication | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 1554-1556 |
| Number of pages | 3 |
| ISBN (Electronic) | 9780979806476 |
| Publication status | Published - 2014 Jan 1 |
| Event | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States Duration: 2014 Oct 26 → 2014 Oct 30 |
Publication series
| Name | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
|---|
Other
| Other | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
|---|---|
| Country | United States |
| City | San Antonio |
| Period | 14-10-26 → 14-10-30 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Control and Systems Engineering
Cite this
}
Rapid and dynamic signal enhancement of bead-based bioassays using a multi-functional optoelectric device. / Wang, K. C.; Li, F. T.; Shieh, Dar-Bin; Kim, K. C.; Chuang, Han-Sheng.
18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. p. 1554-1556 (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
TY - GEN
T1 - Rapid and dynamic signal enhancement of bead-based bioassays using a multi-functional optoelectric device
AU - Wang, K. C.
AU - Li, F. T.
AU - Shieh, Dar-Bin
AU - Kim, K. C.
AU - Chuang, Han-Sheng
PY - 2014/1/1
Y1 - 2014/1/1
N2 - Colloidal particles are broadly used in biochemical applications. Considerable research works have proven particles to be useful carriers in many bead-based bioassays. The increasing use of colloidal systems raises demands for a versatile tool capable of performing multiple functions. To this end, this paper presents a simple optoelectrokinetic method for manipulating particles by simultaneously inducing electrohydrodnamic flow and electrokinetic motions. With carefully tuning the balance between several forces acting on particles, manipulation capabilities including aggregation, translation, sorting, and patterning were achieved. The experimental results showed that the technique is subjected to frequency, electric voltage, laser intensity, and particle size. For a proof of concept, the technique was used to enhance the weak signal of a dilute biotin solution by concentrating streptavidin coated particles. Compared with a ELISA reader, the minimal readout was 100 fold improvement. The demonstration provides a good insight to the future biochemical applications.
AB - Colloidal particles are broadly used in biochemical applications. Considerable research works have proven particles to be useful carriers in many bead-based bioassays. The increasing use of colloidal systems raises demands for a versatile tool capable of performing multiple functions. To this end, this paper presents a simple optoelectrokinetic method for manipulating particles by simultaneously inducing electrohydrodnamic flow and electrokinetic motions. With carefully tuning the balance between several forces acting on particles, manipulation capabilities including aggregation, translation, sorting, and patterning were achieved. The experimental results showed that the technique is subjected to frequency, electric voltage, laser intensity, and particle size. For a proof of concept, the technique was used to enhance the weak signal of a dilute biotin solution by concentrating streptavidin coated particles. Compared with a ELISA reader, the minimal readout was 100 fold improvement. The demonstration provides a good insight to the future biochemical applications.
UR - http://www.scopus.com/inward/record.url?scp=84941651786&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84941651786&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84941651786
T3 - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
SP - 1554
EP - 1556
BT - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PB - Chemical and Biological Microsystems Society
ER -