Rapid and reversible immobilization of caenorhabditis elegans with an optoelectric device and pluronic F-127

Wan Yu Chuang, Han-Sheng Chuang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper reports a development of an optoelectric device with Pluronic (P) F-127 for reversibly immobilizing Caenorhabditis (C.) elegans. The gelation time was less than 5 s, and C. elegans also recovered from the immobilization within 10 s. The proposed device achieved addressable immobilization for long-term observation and imaging with less stress acting on worms. Assessments regarding growth, progeny, lifespan and stress were conducted. Worms imposed with simultaneous laser irradiation and an electric field in the PF-127 solution within 3 h incurred no adverse reactions. The technique is expected to benefit the research work on drug screening in the future.

Original languageEnglish
Title of host publicationMEMS 2016 - 29th IEEE International Conference on Micro Electro Mechanical Systems
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages769-771
Number of pages3
ISBN (Electronic)9781509019731
DOIs
Publication statusPublished - 2016 Feb 26
Event29th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2016 - Shanghai, China
Duration: 2016 Jan 242016 Jan 28

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Volume2016-February
ISSN (Print)1084-6999

Other

Other29th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2016
CountryChina
CityShanghai
Period16-01-2416-01-28

Fingerprint

Poloxamer
worms
immobilization
progeny
gelation
Laser beam effects
Gelation
Screening
drugs
screening
Electric fields
Imaging techniques
irradiation
electric fields
Pharmaceutical Preparations
lasers

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

Cite this

Chuang, W. Y., & Chuang, H-S. (2016). Rapid and reversible immobilization of caenorhabditis elegans with an optoelectric device and pluronic F-127. In MEMS 2016 - 29th IEEE International Conference on Micro Electro Mechanical Systems (pp. 769-771). [7421742] (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS); Vol. 2016-February). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MEMSYS.2016.7421742
Chuang, Wan Yu ; Chuang, Han-Sheng. / Rapid and reversible immobilization of caenorhabditis elegans with an optoelectric device and pluronic F-127. MEMS 2016 - 29th IEEE International Conference on Micro Electro Mechanical Systems. Institute of Electrical and Electronics Engineers Inc., 2016. pp. 769-771 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).
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Chuang, WY & Chuang, H-S 2016, Rapid and reversible immobilization of caenorhabditis elegans with an optoelectric device and pluronic F-127. in MEMS 2016 - 29th IEEE International Conference on Micro Electro Mechanical Systems., 7421742, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), vol. 2016-February, Institute of Electrical and Electronics Engineers Inc., pp. 769-771, 29th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2016, Shanghai, China, 16-01-24. https://doi.org/10.1109/MEMSYS.2016.7421742

Rapid and reversible immobilization of caenorhabditis elegans with an optoelectric device and pluronic F-127. / Chuang, Wan Yu; Chuang, Han-Sheng.

MEMS 2016 - 29th IEEE International Conference on Micro Electro Mechanical Systems. Institute of Electrical and Electronics Engineers Inc., 2016. p. 769-771 7421742 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS); Vol. 2016-February).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Chuang WY, Chuang H-S. Rapid and reversible immobilization of caenorhabditis elegans with an optoelectric device and pluronic F-127. In MEMS 2016 - 29th IEEE International Conference on Micro Electro Mechanical Systems. Institute of Electrical and Electronics Engineers Inc. 2016. p. 769-771. 7421742. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2016.7421742