Rapid and reversible immobilization of caenorhabditis elegans with an optoelectric device and pluronic F-127

Wan Yu Chuang; Han Sheng Chuang

doi:10.1109/MEMSYS.2016.7421742

Rapid and reversible immobilization of caenorhabditis elegans with an optoelectric device and pluronic F-127

Wan Yu Chuang, Han Sheng Chuang

Department of Biomedical Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

This paper reports a development of an optoelectric device with Pluronic (P) F-127 for reversibly immobilizing Caenorhabditis (C.) elegans. The gelation time was less than 5 s, and C. elegans also recovered from the immobilization within 10 s. The proposed device achieved addressable immobilization for long-term observation and imaging with less stress acting on worms. Assessments regarding growth, progeny, lifespan and stress were conducted. Worms imposed with simultaneous laser irradiation and an electric field in the PF-127 solution within 3 h incurred no adverse reactions. The technique is expected to benefit the research work on drug screening in the future.

Original language	English
Title of host publication	MEMS 2016 - 29th IEEE International Conference on Micro Electro Mechanical Systems
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	769-771
Number of pages	3
ISBN (Electronic)	9781509019731
DOIs	https://doi.org/10.1109/MEMSYS.2016.7421742
Publication status	Published - 2016 Feb 26
Event	29th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2016 - Shanghai, China Duration: 2016 Jan 24 → 2016 Jan 28

Publication series

Name	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Volume	2016-February
ISSN (Print)	1084-6999

Other

Other	29th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2016
Country/Territory	China
City	Shanghai
Period	16-01-24 → 16-01-28

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Mechanical Engineering
Electrical and Electronic Engineering

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10.1109/MEMSYS.2016.7421742

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Chuang, W. Y., & Chuang, H. S. (2016). Rapid and reversible immobilization of caenorhabditis elegans with an optoelectric device and pluronic F-127. In MEMS 2016 - 29th IEEE International Conference on Micro Electro Mechanical Systems (pp. 769-771). Article 7421742 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS); Vol. 2016-February). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MEMSYS.2016.7421742

Chuang, Wan Yu ; Chuang, Han Sheng. / Rapid and reversible immobilization of caenorhabditis elegans with an optoelectric device and pluronic F-127. MEMS 2016 - 29th IEEE International Conference on Micro Electro Mechanical Systems. Institute of Electrical and Electronics Engineers Inc., 2016. pp. 769-771 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

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title = "Rapid and reversible immobilization of caenorhabditis elegans with an optoelectric device and pluronic F-127",

abstract = "This paper reports a development of an optoelectric device with Pluronic (P) F-127 for reversibly immobilizing Caenorhabditis (C.) elegans. The gelation time was less than 5 s, and C. elegans also recovered from the immobilization within 10 s. The proposed device achieved addressable immobilization for long-term observation and imaging with less stress acting on worms. Assessments regarding growth, progeny, lifespan and stress were conducted. Worms imposed with simultaneous laser irradiation and an electric field in the PF-127 solution within 3 h incurred no adverse reactions. The technique is expected to benefit the research work on drug screening in the future.",

author = "Chuang, {Wan Yu} and Chuang, {Han Sheng}",

note = "Funding Information: This study was supported by the Ministry of Science and Technology under the grant number 103- 2221- E-006- 172- MY2.; 29th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2016 ; Conference date: 24-01-2016 Through 28-01-2016",

year = "2016",

month = feb,

day = "26",

doi = "10.1109/MEMSYS.2016.7421742",

language = "English",

series = "Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

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Chuang, WY & Chuang, HS 2016, Rapid and reversible immobilization of caenorhabditis elegans with an optoelectric device and pluronic F-127. in MEMS 2016 - 29th IEEE International Conference on Micro Electro Mechanical Systems., 7421742, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), vol. 2016-February, Institute of Electrical and Electronics Engineers Inc., pp. 769-771, 29th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2016, Shanghai, China, 16-01-24. https://doi.org/10.1109/MEMSYS.2016.7421742

Rapid and reversible immobilization of caenorhabditis elegans with an optoelectric device and pluronic F-127. / Chuang, Wan Yu; Chuang, Han Sheng.
MEMS 2016 - 29th IEEE International Conference on Micro Electro Mechanical Systems. Institute of Electrical and Electronics Engineers Inc., 2016. p. 769-771 7421742 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS); Vol. 2016-February).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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N2 - This paper reports a development of an optoelectric device with Pluronic (P) F-127 for reversibly immobilizing Caenorhabditis (C.) elegans. The gelation time was less than 5 s, and C. elegans also recovered from the immobilization within 10 s. The proposed device achieved addressable immobilization for long-term observation and imaging with less stress acting on worms. Assessments regarding growth, progeny, lifespan and stress were conducted. Worms imposed with simultaneous laser irradiation and an electric field in the PF-127 solution within 3 h incurred no adverse reactions. The technique is expected to benefit the research work on drug screening in the future.

AB - This paper reports a development of an optoelectric device with Pluronic (P) F-127 for reversibly immobilizing Caenorhabditis (C.) elegans. The gelation time was less than 5 s, and C. elegans also recovered from the immobilization within 10 s. The proposed device achieved addressable immobilization for long-term observation and imaging with less stress acting on worms. Assessments regarding growth, progeny, lifespan and stress were conducted. Worms imposed with simultaneous laser irradiation and an electric field in the PF-127 solution within 3 h incurred no adverse reactions. The technique is expected to benefit the research work on drug screening in the future.

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Chuang WY, Chuang HS. Rapid and reversible immobilization of caenorhabditis elegans with an optoelectric device and pluronic F-127. In MEMS 2016 - 29th IEEE International Conference on Micro Electro Mechanical Systems. Institute of Electrical and Electronics Engineers Inc. 2016. p. 769-771. 7421742. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). doi: 10.1109/MEMSYS.2016.7421742

Rapid and reversible immobilization of caenorhabditis elegans with an optoelectric device and pluronic F-127

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