Rapid bead-based antimicrobial susceptibility testing by optical diffusometry

Chih Yao Chung, Jhih Cheng Wang, Han-Sheng Chuang

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

This study combined optical diffusometry and bead-based immunoassays to develop a novel technique for quantifying the growth of specific microorganisms and achieving rapid AST. Diffusivity rises when live bacteria attach to particles, resulting in additional energy from motile microorganisms. However, when UV-sterilized (dead) bacteria attach to particles, diffusivity declines. The experimental data are consistent with the theoretical model predicted according to the equivalent volume diameter. Using this diffusometric platform, the susceptibility of Pseudomonas aeruginosa to the antibiotic gentamicin was tested. The result suggests that the proliferation of bacteria is effectively controlled by gentamicin. This study demonstrated a sensitive (one bacterium on single particles) and time-saving (within 2 h) platform with a small sample volume (∼0.5 μL) and a low initial bacteria count (50 CFU per droplet ∼ 105 CFU/mL) for quantifying the growth of microorganisms depending on Brownian motion. The technique can be applied further to other bacterial strains and increase the success of treatments against infectious diseases in the near future.

Original languageEnglish
Article numbere0148864
JournalPloS one
Volume11
Issue number2
DOIs
Publication statusPublished - 2016 Feb 1

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Bacteria
anti-infective agents
Microorganisms
bacteria
Testing
gentamicin
Gentamicins
microorganisms
diffusivity
testing
Brownian movement
Growth
immunoassays
Immunoassay
Pseudomonas aeruginosa
infectious diseases
droplets
Communicable Diseases
Theoretical Models
antibiotics

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

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Rapid bead-based antimicrobial susceptibility testing by optical diffusometry. / Chung, Chih Yao; Wang, Jhih Cheng; Chuang, Han-Sheng.

In: PloS one, Vol. 11, No. 2, e0148864, 01.02.2016.

Research output: Contribution to journalArticle

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