Rapid characterization of sugar-binding specificity by in-solution proximity binding with photosensitizers

Chuan Fa Chang, Jia Fu Pan, Chun Nan Lin, I. Lin Wu, Chi Huey Wong, Chun Hung Lin

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

Cell-surface carbohydrates are known to participate in many important physiological and pathological activities by interacting with their corresponding proteins or receptors. Although several methods have been developed for studying carbohydrate-protein interactions, one major problem originates from the weak bindings of carbohydrates/proteins that are often lost during repeating wash steps. Herein, we established a homogeneous solution carbohydrate array in which polyacrylamide-based glycans are used for offering a multivalent environment. The method requires no wash step and can be carried out in a high-throughput manner. We characterized the carbohydrate-binding specificities of 11 lectins and 7 antibodies, the majority of which displayed the binding patterns in consistence with previous reports. These results demonstrate that our developed solution carbohydrate array provides a useful alternative that is better than or comparable with the current available methods.

Original languageEnglish
Pages (from-to)895-902
Number of pages8
JournalGlycobiology
Volume21
Issue number7
DOIs
Publication statusPublished - 2011 Jul

All Science Journal Classification (ASJC) codes

  • Biochemistry

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