Rapid detection of gut microbial metabolite trimethylamine n-oxide for chronic kidney disease prevention

Yu Chun Chang, Yi Hsuan Chu, Chien Cheng Wang, Chih Hsuan Wang, You Lin Tain, Hung Wei Yang

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)


The gut microbiota plays a critical role in chronic kidney disease (CKD) and hypertension. Trimethylamine-N-oxide (TMAO) and trimethylamine (TMA) are gut microbiota-derived metabolites, and both are known uraemic toxins that are implicated in CKD, atherosclerosis, colorectal cancer and cardiovascular risk. Therefore, the detection and quantification of TMAO, which is a metabolite from gut microbes, are important for the diagnosis of diseases such as atherosclerosis, thrombosis and colorectal cancer. In this study, a new “colour-switch” method that is based on the combination of a plasma separation pad/absorption pad and polyallylamine hydrochloride-capped manganese dioxide (PAH@MnO2 ) nanozyme was developed for the direct quantitative detection of TMAO in whole blood without blood sample pretreatment. As a proof of concept, a limit of quantitation (LOQ) of less than 6.7 µM for TMAO was obtained with a wide linear quantification range from 15.6 to 500 µM through quantitative analysis, thereby suggesting potential clinical applications in blood TMAO monitoring for CKD patients.

Original languageEnglish
Article number339
Issue number9
Publication statusPublished - 2021 Sept

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Instrumentation
  • Engineering (miscellaneous)
  • Biotechnology
  • Biomedical Engineering
  • Clinical Biochemistry


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