Rapid detection of influenza infection with magnetic MnFe2O 4 nanoparticle-based immunoassay by using an integrated microfluidic system

Lien Yu Hung, Gwo Bin Lee, Jui Cheng Chang, Chih-Chia Huang, Chen-Sheng Yeh, Yi Che Tsai, Chih-Peng Chang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this study, new magnetic manganese ferrite (MnFe2O 4) nanoparticles with a size around 100 nanometer (nm) in diameter were used to improve the performance of an immunoassay for detection of influenza infection. A new microfluidic system was developed to automatically implement the entire detection process. In order to apply these new nanoparticles for influenza detection, the design of the micromixer was optimized to reduce the dead volume. Furthermore, a custom-made control system was used for automating the entire chip operation. The synthesized nanoparticles were tested for three months to confirm the stability of the process of thermal decomposition. Furthermore, with the custom-made control system, mixing index of the modified micromixer can be as high as 96% in 2 seconds under both positive and negative air forces under a driving frequency of 4Hz. The optical signals showed that this nanoparticle-based immunoassay could successfully achieve a limit of detection as low as 0.03 Hau. This developed microfluidic system can automatically perform the entire process involved in the immunoassay, including virus purification and detection, and therefore may provide a promising platform for fast diagnosis of the infectious diseases.

Original languageEnglish
Title of host publicationNANOMED 2012 - 6th IEEE International Conference on Nano/Molecular Medicine and Engineering
Pages1-5
Number of pages5
DOIs
Publication statusPublished - 2012 Dec 1
Event6th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2012 - Bangkok, Thailand
Duration: 2012 Nov 42012 Nov 7

Publication series

NameIEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED
ISSN (Print)2159-6964
ISSN (Electronic)2159-6972

Other

Other6th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2012
CountryThailand
CityBangkok
Period12-11-0412-11-07

Fingerprint

Microfluidics
Immunoassay
Nanoparticles
Human Influenza
Infection
Control systems
Viruses
Manganese
Purification
Communicable Diseases
Ferrite
Limit of Detection
Pyrolysis
Hot Temperature
Air

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Biomedical Engineering
  • Microbiology (medical)

Cite this

Hung, L. Y., Lee, G. B., Chang, J. C., Huang, C-C., Yeh, C-S., Tsai, Y. C., & Chang, C-P. (2012). Rapid detection of influenza infection with magnetic MnFe2O 4 nanoparticle-based immunoassay by using an integrated microfluidic system. In NANOMED 2012 - 6th IEEE International Conference on Nano/Molecular Medicine and Engineering (pp. 1-5). [6509133] (IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED). https://doi.org/10.1109/NANOMED.2012.6509133
Hung, Lien Yu ; Lee, Gwo Bin ; Chang, Jui Cheng ; Huang, Chih-Chia ; Yeh, Chen-Sheng ; Tsai, Yi Che ; Chang, Chih-Peng. / Rapid detection of influenza infection with magnetic MnFe2O 4 nanoparticle-based immunoassay by using an integrated microfluidic system. NANOMED 2012 - 6th IEEE International Conference on Nano/Molecular Medicine and Engineering. 2012. pp. 1-5 (IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED).
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abstract = "In this study, new magnetic manganese ferrite (MnFe2O 4) nanoparticles with a size around 100 nanometer (nm) in diameter were used to improve the performance of an immunoassay for detection of influenza infection. A new microfluidic system was developed to automatically implement the entire detection process. In order to apply these new nanoparticles for influenza detection, the design of the micromixer was optimized to reduce the dead volume. Furthermore, a custom-made control system was used for automating the entire chip operation. The synthesized nanoparticles were tested for three months to confirm the stability of the process of thermal decomposition. Furthermore, with the custom-made control system, mixing index of the modified micromixer can be as high as 96{\%} in 2 seconds under both positive and negative air forces under a driving frequency of 4Hz. The optical signals showed that this nanoparticle-based immunoassay could successfully achieve a limit of detection as low as 0.03 Hau. This developed microfluidic system can automatically perform the entire process involved in the immunoassay, including virus purification and detection, and therefore may provide a promising platform for fast diagnosis of the infectious diseases.",
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Hung, LY, Lee, GB, Chang, JC, Huang, C-C, Yeh, C-S, Tsai, YC & Chang, C-P 2012, Rapid detection of influenza infection with magnetic MnFe2O 4 nanoparticle-based immunoassay by using an integrated microfluidic system. in NANOMED 2012 - 6th IEEE International Conference on Nano/Molecular Medicine and Engineering., 6509133, IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED, pp. 1-5, 6th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2012, Bangkok, Thailand, 12-11-04. https://doi.org/10.1109/NANOMED.2012.6509133

Rapid detection of influenza infection with magnetic MnFe2O 4 nanoparticle-based immunoassay by using an integrated microfluidic system. / Hung, Lien Yu; Lee, Gwo Bin; Chang, Jui Cheng; Huang, Chih-Chia; Yeh, Chen-Sheng; Tsai, Yi Che; Chang, Chih-Peng.

NANOMED 2012 - 6th IEEE International Conference on Nano/Molecular Medicine and Engineering. 2012. p. 1-5 6509133 (IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Hung LY, Lee GB, Chang JC, Huang C-C, Yeh C-S, Tsai YC et al. Rapid detection of influenza infection with magnetic MnFe2O 4 nanoparticle-based immunoassay by using an integrated microfluidic system. In NANOMED 2012 - 6th IEEE International Conference on Nano/Molecular Medicine and Engineering. 2012. p. 1-5. 6509133. (IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED). https://doi.org/10.1109/NANOMED.2012.6509133