Rapid identification of bacteria and Candida pathogens in peritoneal dialysis effluent from patients with peritoneal dialysis-related peritonitis by use of multilocus PCR coupled with electrospray ionization mass spectrometry

Yu Tzu Chang, Hsuan Chen Wang, Ming Cheng Wang, An Bang Wu, Junne Ming Sung, H. Sunny Sun, Ih Jen Su, Wei Chih Kan, Chih Chiang Chien, Jyh Chang Hwang, Hsien Yi Wang, Chin Chung Tseng, Chi Jung Wu

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Abstract

PCR coupled with electrospray ionization mass spectrometry (PCR/ESI-MS) was compared with culture for pathogen detection in peritoneal dialysis (PD)-related peritonitis. Of 21 samples of PD effluent, PCR/ESI-MS identified microorganisms in 18 (86%) samples, including Mycobacterium tuberculosis in 1 culture-negative sample. Of 15 double-positive samples, PCR/ESI-MS and culture reached levels of agreement of 100% (15/15) and 87.5% (7/8) at the genus and species levels, respectively. PCR/ESI-MS can be used for rapid pathogen detection in PD-related peritonitis.

Original languageEnglish
Pages (from-to)1217-1219
Number of pages3
JournalJournal of Clinical Microbiology
Volume52
Issue number4
DOIs
Publication statusPublished - 2014 Apr

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All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

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