Rapid identification of bacteria and Candida pathogens in peritoneal dialysis effluent from patients with peritoneal dialysis-related peritonitis by use of multilocus PCR coupled with electrospray ionization mass spectrometry

Yu Tzu Chang; Hsuan Chen Wang; Ming Cheng Wang; An Bang Wu; Junne Ming Sung; H. Sunny Sun; Ih Jen Su; Wei Chih Kan; Chih Chiang Chien; Jyh Chang Hwang; Hsien Yi Wang; Chin Chung Tseng; Chi Jung Wu

doi:10.1128/JCM.03106-13

Rapid identification of bacteria and Candida pathogens in peritoneal dialysis effluent from patients with peritoneal dialysis-related peritonitis by use of multilocus PCR coupled with electrospray ionization mass spectrometry

Yu Tzu Chang, Hsuan Chen Wang, Ming Cheng Wang, An Bang Wu, Junne Ming Sung, H. Sunny Sun, Ih Jen Su, Wei Chih Kan, Chih Chiang Chien, Jyh Chang Hwang, Hsien Yi Wang, Chin Chung Tseng, Chi Jung Wu

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15 Citations (Scopus)

Abstract

PCR coupled with electrospray ionization mass spectrometry (PCR/ESI-MS) was compared with culture for pathogen detection in peritoneal dialysis (PD)-related peritonitis. Of 21 samples of PD effluent, PCR/ESI-MS identified microorganisms in 18 (86%) samples, including Mycobacterium tuberculosis in 1 culture-negative sample. Of 15 double-positive samples, PCR/ESI-MS and culture reached levels of agreement of 100% (15/15) and 87.5% (7/8) at the genus and species levels, respectively. PCR/ESI-MS can be used for rapid pathogen detection in PD-related peritonitis.

Original language	English
Pages (from-to)	1217-1219
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	52
Issue number	4
DOIs	https://doi.org/10.1128/JCM.03106-13
Publication status	Published - 2014 Apr

All Science Journal Classification (ASJC) codes

Microbiology (medical)

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10.1128/JCM.03106-13

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Chang, Y. T., Wang, H. C., Wang, M. C., Wu, A. B., Sung, J. M., Sun, H. S., Su, I. J., Kan, W. C., Chien, C. C., Hwang, J. C., Wang, H. Y., Tseng, C. C., & Wu, C. J. (2014). Rapid identification of bacteria and Candida pathogens in peritoneal dialysis effluent from patients with peritoneal dialysis-related peritonitis by use of multilocus PCR coupled with electrospray ionization mass spectrometry. Journal of Clinical Microbiology, 52(4), 1217-1219. https://doi.org/10.1128/JCM.03106-13

@article{7013782c16ac47caa00228ec463bfb31,

title = "Rapid identification of bacteria and Candida pathogens in peritoneal dialysis effluent from patients with peritoneal dialysis-related peritonitis by use of multilocus PCR coupled with electrospray ionization mass spectrometry",

abstract = "PCR coupled with electrospray ionization mass spectrometry (PCR/ESI-MS) was compared with culture for pathogen detection in peritoneal dialysis (PD)-related peritonitis. Of 21 samples of PD effluent, PCR/ESI-MS identified microorganisms in 18 (86%) samples, including Mycobacterium tuberculosis in 1 culture-negative sample. Of 15 double-positive samples, PCR/ESI-MS and culture reached levels of agreement of 100% (15/15) and 87.5% (7/8) at the genus and species levels, respectively. PCR/ESI-MS can be used for rapid pathogen detection in PD-related peritonitis.",

author = "Chang, {Yu Tzu} and Wang, {Hsuan Chen} and Wang, {Ming Cheng} and Wu, {An Bang} and Sung, {Junne Ming} and Sun, {H. Sunny} and Su, {Ih Jen} and Kan, {Wei Chih} and Chien, {Chih Chiang} and Hwang, {Jyh Chang} and Wang, {Hsien Yi} and Tseng, {Chin Chung} and Wu, {Chi Jung}",

year = "2014",

month = apr,

doi = "10.1128/JCM.03106-13",

language = "English",

volume = "52",

pages = "1217--1219",

journal = "Journal of Clinical Microbiology",

issn = "0095-1137",

publisher = "American Society for Microbiology",

number = "4",

}

Chang, YT, Wang, HC, Wang, MC , Wu, AB , Sung, JM , Sun, HS, Su, IJ, Kan, WC, Chien, CC, Hwang, JC, Wang, HY, Tseng, CC & Wu, CJ 2014, 'Rapid identification of bacteria and Candida pathogens in peritoneal dialysis effluent from patients with peritoneal dialysis-related peritonitis by use of multilocus PCR coupled with electrospray ionization mass spectrometry', Journal of Clinical Microbiology, vol. 52, no. 4, pp. 1217-1219. https://doi.org/10.1128/JCM.03106-13

Rapid identification of bacteria and Candida pathogens in peritoneal dialysis effluent from patients with peritoneal dialysis-related peritonitis by use of multilocus PCR coupled with electrospray ionization mass spectrometry. / Chang, Yu Tzu; Wang, Hsuan Chen; Wang, Ming Cheng et al.
In: Journal of Clinical Microbiology, Vol. 52, No. 4, 04.2014, p. 1217-1219.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Rapid identification of bacteria and Candida pathogens in peritoneal dialysis effluent from patients with peritoneal dialysis-related peritonitis by use of multilocus PCR coupled with electrospray ionization mass spectrometry

AU - Chang, Yu Tzu

AU - Wang, Hsuan Chen

AU - Wang, Ming Cheng

AU - Wu, An Bang

AU - Sung, Junne Ming

AU - Sun, H. Sunny

AU - Su, Ih Jen

AU - Kan, Wei Chih

AU - Chien, Chih Chiang

AU - Hwang, Jyh Chang

AU - Wang, Hsien Yi

AU - Tseng, Chin Chung

AU - Wu, Chi Jung

PY - 2014/4

Y1 - 2014/4

N2 - PCR coupled with electrospray ionization mass spectrometry (PCR/ESI-MS) was compared with culture for pathogen detection in peritoneal dialysis (PD)-related peritonitis. Of 21 samples of PD effluent, PCR/ESI-MS identified microorganisms in 18 (86%) samples, including Mycobacterium tuberculosis in 1 culture-negative sample. Of 15 double-positive samples, PCR/ESI-MS and culture reached levels of agreement of 100% (15/15) and 87.5% (7/8) at the genus and species levels, respectively. PCR/ESI-MS can be used for rapid pathogen detection in PD-related peritonitis.

AB - PCR coupled with electrospray ionization mass spectrometry (PCR/ESI-MS) was compared with culture for pathogen detection in peritoneal dialysis (PD)-related peritonitis. Of 21 samples of PD effluent, PCR/ESI-MS identified microorganisms in 18 (86%) samples, including Mycobacterium tuberculosis in 1 culture-negative sample. Of 15 double-positive samples, PCR/ESI-MS and culture reached levels of agreement of 100% (15/15) and 87.5% (7/8) at the genus and species levels, respectively. PCR/ESI-MS can be used for rapid pathogen detection in PD-related peritonitis.

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SN - 0095-1137

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SP - 1217

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JO - Journal of Clinical Microbiology

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Chang YT, Wang HC, Wang MC , Wu AB , Sung JM , Sun HS et al. Rapid identification of bacteria and Candida pathogens in peritoneal dialysis effluent from patients with peritoneal dialysis-related peritonitis by use of multilocus PCR coupled with electrospray ionization mass spectrometry. Journal of Clinical Microbiology. 2014 Apr;52(4):1217-1219. doi: 10.1128/JCM.03106-13

Rapid identification of bacteria and Candida pathogens in peritoneal dialysis effluent from patients with peritoneal dialysis-related peritonitis by use of multilocus PCR coupled with electrospray ionization mass spectrometry

Abstract

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