Rapid inhibition of protein histidine phosphorylation by UV-irradiation in Xanthomonas oryzae pv. oryzae

Hao-Jen Huang, Shan hua Lin, Bei-Chang Yang, Ching ming Cheng, chen chung Yang, Tsong teh Kuo

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Exposure of Xanthomonas oryzae pv. oryzae cells to 254 nm UV radiation resulted in an alteration of protein phosphorylation. Labelling of the phosphohistidine-containing proteins with molecular masses of 81 and 32 kDa, named p81 and p32, was rapidly reduced following UV irradiation in the early exponential cells, but the decrease was not detected in mid-exponential cells. Mitomycin C, a DNA replication inhibitor, and rifampicin, a drug generally used to inhibit RNA synthesis and DNA replication, were also found to reduce the histidyl phosphorylation. However, this alteration of protein phosphorylation was not hindered by chloramphenicol treatment. A possible role for these histidyl phosphopfoteins in sensing UV light is proposed.

Original languageEnglish
Pages (from-to)189-194
Number of pages6
JournalFEMS Microbiology Letters
Volume134
Issue number2-3
DOIs
Publication statusPublished - 1995 Dec 15

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Genetics

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