Rapid (<5 min) identification of pathogen in human blood by electrokinetic concentration and surface-enhanced raman spectroscopy

I. Fang Cheng, Hsien Chang Chang, Tzu Ying Chen, Chenming Hu, Fu Liang Yang

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75 Citations (Scopus)

Abstract

This study reports a novel microfluidic platform for rapid and long-ranged concentration of rare-pathogen from human blood for subsequent on-chip surface-enhanced Raman spectroscopy (SERS) identification/discrimination of bacteria based on their detected fingerprints. Using a hybrid electrokinetic mechanism, bacteria can be concentrated at the stagnation area on the SERS-active roughened electrode, while blood cells were excluded away from this region at the center of concentric circular electrodes. This electrokinetic approach performs isolation and concentration of bacteria in about three minutes; the density factor is increased approximately a thousand fold in a local area of ∼5000 μm2 from a low bacteria concentration of 5 × 103 CFU/ml. Besides, three genera of bacteria, S. aureus, E. coli, and P. aeruginosa that are found in most of the isolated infections in bacteremia were successfully identified in less than one minute on-chip without the use of any antibody/chemical immobilization and reaction processes.

Original languageEnglish
Article number2365
JournalScientific reports
Volume3
DOIs
Publication statusPublished - 2013 Aug 19

All Science Journal Classification (ASJC) codes

  • General

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