Rapid measurement of AFP using AFP-specific aptamer on a microfluidic chip

Chao Jyun Huang, Hsin I. Lin, Shu Chu Shiesh, Gwo Bin Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

This study presents a new suction-type microfluidic system capable of rapid measurement of alpha-fetoprotein (AFP) by utilizing magnetic bead-based technologies. Two modules, including a suction-type incubator for the magnetic beads to capture AFP and acridinium ester (AE)-labeled anti-AFP antibodies and a microfluidic control module for sample transportation, were integrated into this microfluidic system. With the incorporation of AFP-specific aptamer-conjugated magnetic beads, the target AFP could be recognized and attracted onto the surface of the magnetic beads from the clinical sample. The entire process including two-step incubation and purification process could be automatically performed within 25 min, which is only about 20% of the time required when using a benchtop machine (for about 130 min). Besides, the total sample and reagent volume consumed is only 105 μL, which is significantly less than that required in a large system (410 μL). More importantly, experimental results showed that clinical human serum samples can also be accurately analyzed. The microfluidic system may be promising for point-of-care applications for AFP detection in the future.

Original languageEnglish
Title of host publication2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
Pages874-877
Number of pages4
DOIs
Publication statusPublished - 2012 May 7
Event2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012 - Paris, France
Duration: 2012 Jan 292012 Feb 2

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)1084-6999

Other

Other2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
CountryFrance
CityParis
Period12-01-2912-02-02

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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