TY - GEN
T1 - Rapid microwell prototyping, generation of 3D multicellular cancer aggregates, and emt drug screening
AU - Tu, Ting Yuan
AU - Sun, Wei
AU - Peng, Weng Kung
AU - Wang, Zhe
AU - Huang, Ruby Y.J.
AU - Matsudaira, Paul T.
AU - Thiery, Jean Paul
AU - Kamm, Roger D.
N1 - Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.
PY - 2011
Y1 - 2011
N2 - This work presents a microwell prototyping technique for generating multicellular cancer aggregates in suspension culture for epithelial- mesenchymal-transition (EMT) drug screening. Microwell and aggregate size and geometry were examined in PMMA, PDMS, polystyrene materials materials. Retrieved aggregates were further studied in terms of cell dispersion through drug screening, to identify the effective drug dosage for the inhibition of EMT. Two distinct cell phenotypic behaviors were discovered from Low-attachment-dish- and microwell-generated aggregates under different conditions. Future studies of the two types of aggregates may contribute to the understanding of cancer metastasis.
AB - This work presents a microwell prototyping technique for generating multicellular cancer aggregates in suspension culture for epithelial- mesenchymal-transition (EMT) drug screening. Microwell and aggregate size and geometry were examined in PMMA, PDMS, polystyrene materials materials. Retrieved aggregates were further studied in terms of cell dispersion through drug screening, to identify the effective drug dosage for the inhibition of EMT. Two distinct cell phenotypic behaviors were discovered from Low-attachment-dish- and microwell-generated aggregates under different conditions. Future studies of the two types of aggregates may contribute to the understanding of cancer metastasis.
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M3 - Conference contribution
AN - SCOPUS:84883760178
SN - 9781618395955
T3 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
SP - 82
EP - 84
BT - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
T2 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Y2 - 2 October 2011 through 6 October 2011
ER -