Rapid microwell prototyping, generation of 3D multicellular cancer aggregates, and emt drug screening

Ting Yuan Tu, Wei Sun, Weng Kung Peng, Zhe Wang, Ruby Y.J. Huang, Paul T. Matsudaira, Jean Paul Thiery, Roger D. Kamm

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This work presents a microwell prototyping technique for generating multicellular cancer aggregates in suspension culture for epithelial- mesenchymal-transition (EMT) drug screening. Microwell and aggregate size and geometry were examined in PMMA, PDMS, polystyrene materials materials. Retrieved aggregates were further studied in terms of cell dispersion through drug screening, to identify the effective drug dosage for the inhibition of EMT. Two distinct cell phenotypic behaviors were discovered from Low-attachment-dish- and microwell-generated aggregates under different conditions. Future studies of the two types of aggregates may contribute to the understanding of cancer metastasis.

Original languageEnglish
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages82-84
Number of pages3
Publication statusPublished - 2011
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: 2011 Oct 22011 Oct 6

Publication series

Name15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume1

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CountryUnited States
CitySeattle, WA
Period11-10-0211-10-06

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

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