Rapid movement of axonal neurofilaments interrupted by prolonged pauses

Lei Wang, Chung-Liang Ho, Dongming Sun, Ronald K.H. Liem, Anthony Brown

Research output: Contribution to journalArticle

254 Citations (Scopus)

Abstract

Axonal cytoskeletal and cytosolic proteins are synthesized in the neuronal cell body and transported along axons by slow axonal transport, but attempts to observe this movement directly in living cells have yielded conflicting results. Here we report the direct observation of the axonal transport of neurofilament protein tagged with green fluorescent protein in cultured nerve cells. Live-cell imaging of naturally occurring gaps in the axonal neurofilament array reveals rapid, intermittent and highly asynchronous movement of fluorescent neurofilaments. The movement is bidirectional, but predominantly anterograde. Our data indicate that the slow rate of slow axonal transport may be the result of rapid movements interrupted by prolonged pauses.

Original languageEnglish
Pages (from-to)137-141
Number of pages5
JournalNature Cell Biology
Volume2
Issue number3
DOIs
Publication statusPublished - 2000 Oct 30

All Science Journal Classification (ASJC) codes

  • Cell Biology

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