Rapid screening of lignans from Phyllanthus myrtifolius and stilbenoids from Syagrus romanzoffiana by HPLC-SPE-NMR

Chen Yu Wang, Sio-Hong Lam, Li Hong Tseng, Shoei Sheng Lee

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Introduction - Application of on-line solid-phase extraction (SPE) as an interface between HPLC and NMR has gained great improvement in solving sensitivity problems and signal interferences by the eluents. Objective - Rapid analysis and characterisation by HPLC-SPE-NMR and LC/MS of the arylnaphthalene-type lignans present in Phyllanthus myrtifolius and the minor stilbenoids present in the polyphenol-rich fraction from the ethanol extract of the seeds of Syagrus romanzoffiana. Methodology - Pretreatment of fractions by liquid-liquid partitioning, followed by Sephadex LH-20 fractionation, was found very useful to facilitate the focusing and analysis of the polyphenolic fraction. HPLC-DAD-SPE-NMR (400 MHz and 600 MHz) analysis was carried out using an Agilent 1100 liquid chromatography, followed by a Prospekt 2 automated solid-phase extraction unit, containing 96 HySphere-Resin GP cartridges (10 × 2 mm, 10-12 μm), which was connected to a 120 or 60 μL LC probe. Results - Seven arylnaphthalene-type lignans from the chloroform-soluble fraction of P. myrtifolius and nine stilbenoids from a polyphenol-rich butanol-soluble fraction of the seeds of S. romanzoffiana were characterised. Conclusion - HPLC-SPE-NMR associated with HR-ESI/MS, which consumed only analytical amounts of partially purified mixtures, was demonstrated to be a good tool for rapid screening of both known and new natural products.

Original languageEnglish
Pages (from-to)352-360
Number of pages9
JournalPhytochemical Analysis
Volume22
Issue number4
DOIs
Publication statusPublished - 2011 Jul 1

Fingerprint

Syagrus romanzoffiana
Phyllanthus
stilbenoids
Lignans
Solid Phase Extraction
lignans
solid phase extraction
Screening
high performance liquid chromatography
High Pressure Liquid Chromatography
Nuclear magnetic resonance
screening
polyphenols
Polyphenols
Seed
Seeds
Butanols
liquids
seed extracts
Liquid chromatography

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Food Science
  • Biochemistry
  • Molecular Medicine
  • Plant Science
  • Drug Discovery
  • Complementary and alternative medicine

Cite this

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abstract = "Introduction - Application of on-line solid-phase extraction (SPE) as an interface between HPLC and NMR has gained great improvement in solving sensitivity problems and signal interferences by the eluents. Objective - Rapid analysis and characterisation by HPLC-SPE-NMR and LC/MS of the arylnaphthalene-type lignans present in Phyllanthus myrtifolius and the minor stilbenoids present in the polyphenol-rich fraction from the ethanol extract of the seeds of Syagrus romanzoffiana. Methodology - Pretreatment of fractions by liquid-liquid partitioning, followed by Sephadex LH-20 fractionation, was found very useful to facilitate the focusing and analysis of the polyphenolic fraction. HPLC-DAD-SPE-NMR (400 MHz and 600 MHz) analysis was carried out using an Agilent 1100 liquid chromatography, followed by a Prospekt 2 automated solid-phase extraction unit, containing 96 HySphere-Resin GP cartridges (10 × 2 mm, 10-12 μm), which was connected to a 120 or 60 μL LC probe. Results - Seven arylnaphthalene-type lignans from the chloroform-soluble fraction of P. myrtifolius and nine stilbenoids from a polyphenol-rich butanol-soluble fraction of the seeds of S. romanzoffiana were characterised. Conclusion - HPLC-SPE-NMR associated with HR-ESI/MS, which consumed only analytical amounts of partially purified mixtures, was demonstrated to be a good tool for rapid screening of both known and new natural products.",
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Rapid screening of lignans from Phyllanthus myrtifolius and stilbenoids from Syagrus romanzoffiana by HPLC-SPE-NMR. / Wang, Chen Yu; Lam, Sio-Hong; Tseng, Li Hong; Lee, Shoei Sheng.

In: Phytochemical Analysis, Vol. 22, No. 4, 01.07.2011, p. 352-360.

Research output: Contribution to journalArticle

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N2 - Introduction - Application of on-line solid-phase extraction (SPE) as an interface between HPLC and NMR has gained great improvement in solving sensitivity problems and signal interferences by the eluents. Objective - Rapid analysis and characterisation by HPLC-SPE-NMR and LC/MS of the arylnaphthalene-type lignans present in Phyllanthus myrtifolius and the minor stilbenoids present in the polyphenol-rich fraction from the ethanol extract of the seeds of Syagrus romanzoffiana. Methodology - Pretreatment of fractions by liquid-liquid partitioning, followed by Sephadex LH-20 fractionation, was found very useful to facilitate the focusing and analysis of the polyphenolic fraction. HPLC-DAD-SPE-NMR (400 MHz and 600 MHz) analysis was carried out using an Agilent 1100 liquid chromatography, followed by a Prospekt 2 automated solid-phase extraction unit, containing 96 HySphere-Resin GP cartridges (10 × 2 mm, 10-12 μm), which was connected to a 120 or 60 μL LC probe. Results - Seven arylnaphthalene-type lignans from the chloroform-soluble fraction of P. myrtifolius and nine stilbenoids from a polyphenol-rich butanol-soluble fraction of the seeds of S. romanzoffiana were characterised. Conclusion - HPLC-SPE-NMR associated with HR-ESI/MS, which consumed only analytical amounts of partially purified mixtures, was demonstrated to be a good tool for rapid screening of both known and new natural products.

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