Rapid whole-cell sensing chip for low-level arsenite detection

Chi Han Chiou, Liang Ju Chien, Tsung Che Chou, Jr Lung Lin, Ta-Chien Tseng

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

A novel whole-cell sensing chip system consisted of a micro-concentrator, a set of electrochemical detection electrodes, and a microfluidic channel was developed for rapid detection of arsenite in water. Firstly, the E. coli cells transformed with arsenited-regulated reporter plasmids were incubated with solution contained arsenite. Under this condition, the level of reporter protein, β-galactosidase, expressed by E. coli cells is dependent on the concentration of arsenite. Using the dielectrophoretic force, the micro-concentrator continuously enriched the E. coli cells into a small area above the embedded detection electrodes. And then the relative expression levels of β-galactosidase were obtained using the electrochemical method to measure the amount of p-aminophenol (PAP) which converted from the p-aminophenyl-β-. d-galactopyranoside (PAPG) by β-galactosidase. From the result, it indicates this device can detect as low as 0.1. ppm of arsenite within 30. min. Compared with other traditional detection methods, our new device provides better performance like higher sensitivity, shorter analysis time, and lower cost in detecting the arsenite.

Original languageEnglish
Pages (from-to)2484-2488
Number of pages5
JournalBiosensors and Bioelectronics
Volume26
Issue number5
DOIs
Publication statusPublished - 2011 Jan 15

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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