The phylogenetic relationship between Cunninghamia konishii and C. lanceolata was investigated by a cladistic analysis and a minimum spanning network reconstruction on RFLP of the trnD - trnT non-coding spacer of chloroplast DNA. Four sources of C. lanceolata from southeastern provinces of mainland China and 4 sources of C. konishii from Taiwan were sampled. Restriction enzyme digestion with Taq I and Hinf I revealed genetic polymorphisms among populations and identified 4 haplotypes. Monophyly of each species was not supported. A monophyletic group identified by PAUP, with a bootstrap value of 0.70, consists of 3 samples from Taiwan and a sample from Zhejiang Prov. This clade is characterized by the most frequent haplotype, which may represent an ancestral genotype. A reconstructed network revealed a phylogenetic relationship of the Fujian sample linking it to the most frequent type with a loss of a Hinf I site. Another Hinf I site was lost in samples of Hubei and Jiangxi Prov. as well as the Tachien sample of Taiwan, which then gained another Taq I recognition site. Identity between C. konishii and C. lanceolata was suggested based on the RFLP patterns of the chloroplast trnD-trnT spacer.
|Number of pages||7|
|Journal||Taiwan Journal of Forest Science|
|Publication status||Published - 1999 Dec 1|
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