TY - JOUR
T1 - Reactive oxygen species, calcium and serine/threonine phosphatase are required for copper-induced MAP kinase gene, OsMAPK2, expression in rice
AU - Hung, Wan Chi
AU - Huang, Dinq Ding
AU - Yeh, Chuan Ming
AU - Huang, Hao Jen
N1 - Funding Information:
We are deeply grateful to Professor Toshio Murashige for critical reading of the manuscript. This work was supported by research grants from National Science Council and the Ministry of Education of the Republic of China.
PY - 2005/3
Y1 - 2005/3
N2 - Regulation of the MAP kinase gene (OsMAPK2) expression in rice under metal, copper, stress conditions was investigated. OsMAPK2 transcript accumulation was enhanced by copper and H2O2 in rice root-tip cells. To investigate how the OsMAPK2 gene is regulated in response to copper, inhibitor studies were performed. Using Northern blot analysis, it was shown that antioxidant agent (GSH), calcium chelator (EGTA), plasma membrane calcium channel blocker (La3+) and protein phosphatase inhibitor (cantharidin) inhibited copper-induced OsMAPK2 gene activation. We suggest that the stimulation of OsMAPK2 transcript accumulation by copper in rice roots operates may through an intracellular signalling cascade mediated by reactive oxygen species, extracellular calcium ions and cantharidin-sensitive protein phosphatase(s).
AB - Regulation of the MAP kinase gene (OsMAPK2) expression in rice under metal, copper, stress conditions was investigated. OsMAPK2 transcript accumulation was enhanced by copper and H2O2 in rice root-tip cells. To investigate how the OsMAPK2 gene is regulated in response to copper, inhibitor studies were performed. Using Northern blot analysis, it was shown that antioxidant agent (GSH), calcium chelator (EGTA), plasma membrane calcium channel blocker (La3+) and protein phosphatase inhibitor (cantharidin) inhibited copper-induced OsMAPK2 gene activation. We suggest that the stimulation of OsMAPK2 transcript accumulation by copper in rice roots operates may through an intracellular signalling cascade mediated by reactive oxygen species, extracellular calcium ions and cantharidin-sensitive protein phosphatase(s).
UR - https://www.scopus.com/pages/publications/22144467380
UR - https://www.scopus.com/pages/publications/22144467380#tab=citedBy
U2 - 10.1007/s10725-005-1435-3
DO - 10.1007/s10725-005-1435-3
M3 - Article
AN - SCOPUS:22144467380
SN - 0167-6903
VL - 45
SP - 233
EP - 241
JO - Plant Growth Regulation
JF - Plant Growth Regulation
IS - 3
ER -