Reappraisal of HLA Antibody Analysis and Crossmatching in Kidney Transplantation

P. C. Lee; M. Ozawa; C. J. Hung; Y. J. Lin; S. S. Chang; T. C. Chou

doi:10.1016/j.transproceed.2008.10.074

Reappraisal of HLA Antibody Analysis and Crossmatching in Kidney Transplantation

P. C. Lee, M. Ozawa, C. J. Hung, Y. J. Lin, S. S. Chang, T. C. Chou

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

Enzyme-linked immunosorbent assay (ELISA) and flow cytometric techniques have been introduced to overcome the limited sensitivity and specificity of the CDC assay. This retrospective study used lambda antigen tray-mixed screening and Luminex HLA class I and II specificity assays to re-examine: (1) the accuracy with which detection of HLA antibody and specificity by ELISA predicts pretransplantation National Institutes of Health (NIH)/Centers for Disease Control and Prevention (CDC) crossmatch; and (2) a comparison of Luminex and ELISA methods to detect HLA antibodies. Sera from 481 patients awaiting kidney transplantation were tested using the ELISA method lambda antigen tray-mixed and using NIH-CDC to determine how well HLA antibodies detected using ELISA predicted crossmatches using CDC. Pretransplantation sera from 48 patients with follow-up data were retested using both ELISA lambda antigen tray-mixed and Luminex to compare the efficacy of the 2 methods.

Original language	English
Pages (from-to)	95-98
Number of pages	4
Journal	Transplantation Proceedings
Volume	41
Issue number	1
DOIs	https://doi.org/10.1016/j.transproceed.2008.10.074
Publication status	Published - 2009 Jan

All Science Journal Classification (ASJC) codes

Surgery
Transplantation

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10.1016/j.transproceed.2008.10.074

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AU - Chou, T. C.

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Reappraisal of HLA Antibody Analysis and Crossmatching in Kidney Transplantation

Abstract

All Science Journal Classification (ASJC) codes

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