Reclamation of squid pen by Bacillus licheniformis TKU004 for the production of thermally stable and antimicrobial biosurfactant

Yu Chi Chen, Tsung Ju Chiang, Tzu Wen Liang, I. Li Wang, San Lang Wang

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Bacillus licheniformis TKU004 produced biosurfactants in a medium with 1% squid pen powder as the sole carbon/nitrogen source at 37 °C for 1 day by using a baffled base flask, showing a growth-associated production. The biosurfactant was extracted from the culture supernatant by acid precipitation, extraction of ethyl acetate and methanol with the yield of 0.55. g/L. The biosurfactant could reduce surface tension of water from 72.30 to 25.42. mN/m at a concentration of 350. mg/L and the emulsifi{ligature}cation index of machine oil reached 68% after 24. h. The biosurfactant showed stability including thermal (121 °C, 20. min) and pH (7-11) stability regarding the surface tension reduction and the emulsifi{ligature}cation capacity of machine oil, and tolerance under salt concentrations (<2%). The biosurfactant also showed antimicrobial activity significantly against cell growth of E. coli BCRC13086 and S. aureus BCRC10780. The results obtained in this work are noteworthy for possible biosurfactant production from SPP with potential of application in removal of oil and in the future to gasoline-contaminated ocean shores for ecological protection purposes. Besides, it is also possible to be applied as a biocontrol agent.

Original languageEnglish
Pages (from-to)62-69
Number of pages8
JournalBiocatalysis and Agricultural Biotechnology
Volume1
Issue number1
DOIs
Publication statusPublished - 2012 Jan 1

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Agronomy and Crop Science

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