TY - JOUR
T1 - Recognition of molecularly imprinted polymers for a quaternary alkaloid of berberine
AU - Chen, Chia-Yun
AU - Wang, Chih Hung
AU - Chen, Arh Hwang
PY - 2011/5/30
Y1 - 2011/5/30
N2 - Selective and affinitive imprinted polymers incorporating a quaternary alkaloid of berberine (BER) were prepared using a non-covalent imprinting method. The results showed that, compared to other imprinted polymers, the polymer AD-10 had not only a higher of the ratio of QMIP/Q BP for BER adsorption, and but also a larger of the ratio of Q MIP,B/QMIP,P for BER and palmatine (PAL) adsorptions. Spectrophotometric analysis demonstrated that a 1:1 cooperative hydrogen-bonding complex might be predominating in the pre-polymerization between the BER template and AA monomer. Adsorption experiments of BER on the polymer AD-10 were in accordance with the second-order and Langmuir adsorption models. The E value (5.70 kJ/mol) calculated from the Dubinin-Radushkevich model indicated that the adsorption followed a physisorption process. In addition, a Scatchard plot showed a single straight line with an equilibrium dissociation constant (K D) of 65.80 μmol/L. SPE analyses of a mixture of BER and PAL and the methanol extract from the cortices of Phellodendron wilsonii showed that AD-10 had more efficiency, and higher specificity and selectivity for SPE in the concentration and determination of BER and its extraction from natural products.
AB - Selective and affinitive imprinted polymers incorporating a quaternary alkaloid of berberine (BER) were prepared using a non-covalent imprinting method. The results showed that, compared to other imprinted polymers, the polymer AD-10 had not only a higher of the ratio of QMIP/Q BP for BER adsorption, and but also a larger of the ratio of Q MIP,B/QMIP,P for BER and palmatine (PAL) adsorptions. Spectrophotometric analysis demonstrated that a 1:1 cooperative hydrogen-bonding complex might be predominating in the pre-polymerization between the BER template and AA monomer. Adsorption experiments of BER on the polymer AD-10 were in accordance with the second-order and Langmuir adsorption models. The E value (5.70 kJ/mol) calculated from the Dubinin-Radushkevich model indicated that the adsorption followed a physisorption process. In addition, a Scatchard plot showed a single straight line with an equilibrium dissociation constant (K D) of 65.80 μmol/L. SPE analyses of a mixture of BER and PAL and the methanol extract from the cortices of Phellodendron wilsonii showed that AD-10 had more efficiency, and higher specificity and selectivity for SPE in the concentration and determination of BER and its extraction from natural products.
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U2 - 10.1016/j.talanta.2011.03.009
DO - 10.1016/j.talanta.2011.03.009
M3 - Article
C2 - 21530777
AN - SCOPUS:79955523727
VL - 84
SP - 1038
EP - 1046
JO - Talanta
JF - Talanta
SN - 0039-9140
IS - 4
ER -