Recombinant derp5 allergen with αS1-casein signal peptide secreted in murine milk protects against dust mite allergen-induced airway inflammation

Hsu Chung Liu, Shun Yuan Pai, Hsiao Ling Chen, Cheng Wei Lai, Tung Chou Tsai, Winston T.K. Cheng, Shang-Hsun Yang, Chuan Mu Chen

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Recent advances in recombinant technology make transgenic animals that produce pharmaceutical proteins in their milk more feasible. The group 5 allergen isolated from Dermatophagoides pteronyssinus (Derp5) is one of the most important dust mite allergens in humans. The aims of this study were to develop transgenic mice that could secrete recombinant Derp5-containing milk and to demonstrate that ingesting recombinant milk protects against allergic airway inflammation. Two transgenes were constructed separately. The α-LA-Derp5f transgene consisted of the bovine α-lactalbumin (α-LA) promoter and full-length Derp5 cDNA. The α-LA-CN-Derp5t transgene included the α-LA promoter, a leader sequence of αS1-casein (CN), and signal peptide-truncated Derp5 cDNA. Both species of transgenic mice were confirmed to have successful transgene integration and stable germline transmission. Western blot analysis of the milk obtained from the offspring of transgenic mice demonstrated that recombinant Derp5 was secreted successfully in the milk of αLA-CN-Derp5t transgenic mice but not in that of αLA-Derp5f transgenic mice. This study provides new evidence that transgenic mice can secrete recombinant Derp5 efficiently in milk by adding a signal peptide of αS1-casein. The antigenic activity of recombinant Derp5 milk was demonstrated to have a protective effect against allergic airway inflammation in a murine model in which the ingestion of recombinant Derp5-containing milk was used as pretreatment.

Original languageEnglish
Pages (from-to)6792-6803
Number of pages12
JournalJournal of Dairy Science
Volume97
Issue number11
DOIs
Publication statusPublished - 2014 Nov 1

Fingerprint

dust mites
Mites
Protein Sorting Signals
Caseins
allergens
signal peptide
Dust
Allergens
casein
Milk
inflammation
Transgenic Mice
Inflammation
milk
genetically modified organisms
mice
transgenes
Transgenes
promoter regions
Dermatophagoides pteronyssinus

All Science Journal Classification (ASJC) codes

  • Food Science
  • Animal Science and Zoology
  • Genetics

Cite this

Liu, Hsu Chung ; Pai, Shun Yuan ; Chen, Hsiao Ling ; Lai, Cheng Wei ; Tsai, Tung Chou ; Cheng, Winston T.K. ; Yang, Shang-Hsun ; Chen, Chuan Mu. / Recombinant derp5 allergen with αS1-casein signal peptide secreted in murine milk protects against dust mite allergen-induced airway inflammation. In: Journal of Dairy Science. 2014 ; Vol. 97, No. 11. pp. 6792-6803.
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Recombinant derp5 allergen with αS1-casein signal peptide secreted in murine milk protects against dust mite allergen-induced airway inflammation. / Liu, Hsu Chung; Pai, Shun Yuan; Chen, Hsiao Ling; Lai, Cheng Wei; Tsai, Tung Chou; Cheng, Winston T.K.; Yang, Shang-Hsun; Chen, Chuan Mu.

In: Journal of Dairy Science, Vol. 97, No. 11, 01.11.2014, p. 6792-6803.

Research output: Contribution to journalArticle

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