Recovery of gold from industrial wastewater by immobilized gold-binding proteins on porous silica carriers grafted with amino group

Yin Lung Han, Pei Jyuan Gao, Chieh Lun Cheng, Pong Yee Wu, Jo Shu Chang

Research output: Contribution to journalArticle

Abstract

Our recent work demonstrated excellent gold biosorption potential of extracellular proteins obtained from a thermophilic bacterium Tepidimonas fonticaldi AT-A2. In this study, the gold-binding proteins were immobilized on amino-grafted porous silica carriers to further enhance the feasibility of using them for Au recovery from real industrial wastewater. The maximum protein immobilization capacity on the amino-grafted porous silica reached the highest level of 13.83 mg protein/g carrier when the protein loading concentration was 400 mg/L and the amino groups content on the silica carrier was 16%. The mechanical strength of the amino-grafted porous silica could be improved to 19.47 kg by converting the silica powder into pellet form using a calcination temperature of 700 °C. Continuous biosorption of Au (III) from prepared Au solution and from real industrial wastewater was carried out using fixed beds packed with the protein-loaded amino-grafted porous silica pellets. The maximum Au adsorption capacity of the fixed beds with a feeding rate of 0.12 ml/min was 1259 and 448 mg Au/mg protein from the prepared Au solution and an electroplating wastewater (Au concentration was approximately 40 mg/L), respectively. The maximum Au adsorption rate of the fixed beds when using the real wastewater was 33.4 mg/g/h, which is similar to the results of using the prepared Au solution.

Original languageEnglish
Article number107388
JournalBiochemical Engineering Journal
Volume152
DOIs
Publication statusPublished - 2019 Dec 15

Fingerprint

Waste Water
Silicon Dioxide
Gold
Carrier Proteins
Wastewater
Silica
Recovery
Proteins
Biosorption
Adsorption
Electroplating
Packed beds
varespladib methyl
Immobilization
Powders
Calcination
Strength of materials
Bacteria
Temperature

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Environmental Engineering
  • Bioengineering
  • Biomedical Engineering

Cite this

@article{9552f5bfc72a419f8d3034b61bec1d65,
title = "Recovery of gold from industrial wastewater by immobilized gold-binding proteins on porous silica carriers grafted with amino group",
abstract = "Our recent work demonstrated excellent gold biosorption potential of extracellular proteins obtained from a thermophilic bacterium Tepidimonas fonticaldi AT-A2. In this study, the gold-binding proteins were immobilized on amino-grafted porous silica carriers to further enhance the feasibility of using them for Au recovery from real industrial wastewater. The maximum protein immobilization capacity on the amino-grafted porous silica reached the highest level of 13.83 mg protein/g carrier when the protein loading concentration was 400 mg/L and the amino groups content on the silica carrier was 16{\%}. The mechanical strength of the amino-grafted porous silica could be improved to 19.47 kg by converting the silica powder into pellet form using a calcination temperature of 700 °C. Continuous biosorption of Au (III) from prepared Au solution and from real industrial wastewater was carried out using fixed beds packed with the protein-loaded amino-grafted porous silica pellets. The maximum Au adsorption capacity of the fixed beds with a feeding rate of 0.12 ml/min was 1259 and 448 mg Au/mg protein from the prepared Au solution and an electroplating wastewater (Au concentration was approximately 40 mg/L), respectively. The maximum Au adsorption rate of the fixed beds when using the real wastewater was 33.4 mg/g/h, which is similar to the results of using the prepared Au solution.",
author = "Han, {Yin Lung} and Gao, {Pei Jyuan} and Cheng, {Chieh Lun} and Wu, {Pong Yee} and Chang, {Jo Shu}",
year = "2019",
month = "12",
day = "15",
doi = "10.1016/j.bej.2019.107388",
language = "English",
volume = "152",
journal = "Biochemical Engineering Journal",
issn = "1369-703X",
publisher = "Elsevier",

}

Recovery of gold from industrial wastewater by immobilized gold-binding proteins on porous silica carriers grafted with amino group. / Han, Yin Lung; Gao, Pei Jyuan; Cheng, Chieh Lun; Wu, Pong Yee; Chang, Jo Shu.

In: Biochemical Engineering Journal, Vol. 152, 107388, 15.12.2019.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Recovery of gold from industrial wastewater by immobilized gold-binding proteins on porous silica carriers grafted with amino group

AU - Han, Yin Lung

AU - Gao, Pei Jyuan

AU - Cheng, Chieh Lun

AU - Wu, Pong Yee

AU - Chang, Jo Shu

PY - 2019/12/15

Y1 - 2019/12/15

N2 - Our recent work demonstrated excellent gold biosorption potential of extracellular proteins obtained from a thermophilic bacterium Tepidimonas fonticaldi AT-A2. In this study, the gold-binding proteins were immobilized on amino-grafted porous silica carriers to further enhance the feasibility of using them for Au recovery from real industrial wastewater. The maximum protein immobilization capacity on the amino-grafted porous silica reached the highest level of 13.83 mg protein/g carrier when the protein loading concentration was 400 mg/L and the amino groups content on the silica carrier was 16%. The mechanical strength of the amino-grafted porous silica could be improved to 19.47 kg by converting the silica powder into pellet form using a calcination temperature of 700 °C. Continuous biosorption of Au (III) from prepared Au solution and from real industrial wastewater was carried out using fixed beds packed with the protein-loaded amino-grafted porous silica pellets. The maximum Au adsorption capacity of the fixed beds with a feeding rate of 0.12 ml/min was 1259 and 448 mg Au/mg protein from the prepared Au solution and an electroplating wastewater (Au concentration was approximately 40 mg/L), respectively. The maximum Au adsorption rate of the fixed beds when using the real wastewater was 33.4 mg/g/h, which is similar to the results of using the prepared Au solution.

AB - Our recent work demonstrated excellent gold biosorption potential of extracellular proteins obtained from a thermophilic bacterium Tepidimonas fonticaldi AT-A2. In this study, the gold-binding proteins were immobilized on amino-grafted porous silica carriers to further enhance the feasibility of using them for Au recovery from real industrial wastewater. The maximum protein immobilization capacity on the amino-grafted porous silica reached the highest level of 13.83 mg protein/g carrier when the protein loading concentration was 400 mg/L and the amino groups content on the silica carrier was 16%. The mechanical strength of the amino-grafted porous silica could be improved to 19.47 kg by converting the silica powder into pellet form using a calcination temperature of 700 °C. Continuous biosorption of Au (III) from prepared Au solution and from real industrial wastewater was carried out using fixed beds packed with the protein-loaded amino-grafted porous silica pellets. The maximum Au adsorption capacity of the fixed beds with a feeding rate of 0.12 ml/min was 1259 and 448 mg Au/mg protein from the prepared Au solution and an electroplating wastewater (Au concentration was approximately 40 mg/L), respectively. The maximum Au adsorption rate of the fixed beds when using the real wastewater was 33.4 mg/g/h, which is similar to the results of using the prepared Au solution.

UR - http://www.scopus.com/inward/record.url?scp=85072679011&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85072679011&partnerID=8YFLogxK

U2 - 10.1016/j.bej.2019.107388

DO - 10.1016/j.bej.2019.107388

M3 - Article

AN - SCOPUS:85072679011

VL - 152

JO - Biochemical Engineering Journal

JF - Biochemical Engineering Journal

SN - 1369-703X

M1 - 107388

ER -