Regeneration of rete ridges in Lanyu pig (Sus scrofa): Insights for human skin wound healing

Chein Hong Lin, Po Yuan Chiu, Yuan-Yu Hsueh, Shyh-Jou Shieh, Chia-Ching Wu, Tak-Wah Wong, Cheng Ming Chuong, Michael Warren Hughes

Research output: Contribution to journalArticle

Abstract

Rete ridges are important to the mechanical function of skin in animals with minimal hair, including humans. As mice do not exhibit rete ridges, the need for a quality animal model is pertinent. Here, we develop a Lanyu pig (Sus scrofa) full-thickness wound model to explore tissue regeneration because the architecture and function are similar to humans and inbred genetic variants are available. Full- and partial-thickness wounds were generated on the dorsum. Full-thickness wounds at post-wound day 57 exhibit severe scar with no signs of wound-induced hair follicle neogenesis. Wound contraction is greater in the anterior/posterior relative to the medial/lateral axis. In wound beds, K14 + cells increased while K10 + , p63 + and PCNA + cells decreased compared to unwounded tissue. Epithelial β-catenin is unchanged. The wound bed expresses more ColI, less ColIII and no elastin. Rete ridges do not form after full-thickness wounding, but incompletely regenerate after partial-thickness wounding. An alkaline phosphatase (ALP) + cell population, not associated with hair follicles, is present at the bottom of the rete ridge basal layer in pig and human unwounded skin. These K5 + /K10 /PCNA /ALP + epithelial cells are absent after full-thickness wounding but reappear after partial-thickness wounding, before invagination of new rete ridges. In summary, full-thickness wounding on the dorsum of Lanyu pigs results in scar formation and perturbed molecular expression while partial-thickness wounding permits limited rete ridge and papillary dermis regeneration. Future functional studies and further characterization will help contribute knowledge for the regenerative medicine field.

Original languageEnglish
Pages (from-to)472-479
Number of pages8
JournalExperimental Dermatology
Volume28
Issue number4
DOIs
Publication statusPublished - 2019 Apr 1

Fingerprint

Sus scrofa
Proliferating Cell Nuclear Antigen
Wound Healing
Alkaline Phosphatase
Regeneration
Skin
Animals
Swine
Catenins
Tissue regeneration
Elastin
Wounds and Injuries
Cells
Tissue
Hair Follicle
Cicatrix
Regenerative Medicine
Medical Genetics
Dermis
Hair

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Dermatology

Cite this

@article{b49e7d5a88ce47bfbc4cb8d76d66f60c,
title = "Regeneration of rete ridges in Lanyu pig (Sus scrofa): Insights for human skin wound healing",
abstract = "Rete ridges are important to the mechanical function of skin in animals with minimal hair, including humans. As mice do not exhibit rete ridges, the need for a quality animal model is pertinent. Here, we develop a Lanyu pig (Sus scrofa) full-thickness wound model to explore tissue regeneration because the architecture and function are similar to humans and inbred genetic variants are available. Full- and partial-thickness wounds were generated on the dorsum. Full-thickness wounds at post-wound day 57 exhibit severe scar with no signs of wound-induced hair follicle neogenesis. Wound contraction is greater in the anterior/posterior relative to the medial/lateral axis. In wound beds, K14 + cells increased while K10 + , p63 + and PCNA + cells decreased compared to unwounded tissue. Epithelial β-catenin is unchanged. The wound bed expresses more ColI, less ColIII and no elastin. Rete ridges do not form after full-thickness wounding, but incompletely regenerate after partial-thickness wounding. An alkaline phosphatase (ALP) + cell population, not associated with hair follicles, is present at the bottom of the rete ridge basal layer in pig and human unwounded skin. These K5 + /K10 − /PCNA − /ALP + epithelial cells are absent after full-thickness wounding but reappear after partial-thickness wounding, before invagination of new rete ridges. In summary, full-thickness wounding on the dorsum of Lanyu pigs results in scar formation and perturbed molecular expression while partial-thickness wounding permits limited rete ridge and papillary dermis regeneration. Future functional studies and further characterization will help contribute knowledge for the regenerative medicine field.",
author = "Lin, {Chein Hong} and Chiu, {Po Yuan} and Yuan-Yu Hsueh and Shyh-Jou Shieh and Chia-Ching Wu and Tak-Wah Wong and Chuong, {Cheng Ming} and Hughes, {Michael Warren}",
year = "2019",
month = "4",
day = "1",
doi = "10.1111/exd.13875",
language = "English",
volume = "28",
pages = "472--479",
journal = "Experimental Dermatology",
issn = "0906-6705",
publisher = "Wiley-Blackwell",
number = "4",

}

Regeneration of rete ridges in Lanyu pig (Sus scrofa) : Insights for human skin wound healing. / Lin, Chein Hong; Chiu, Po Yuan; Hsueh, Yuan-Yu; Shieh, Shyh-Jou; Wu, Chia-Ching; Wong, Tak-Wah; Chuong, Cheng Ming; Hughes, Michael Warren.

In: Experimental Dermatology, Vol. 28, No. 4, 01.04.2019, p. 472-479.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Regeneration of rete ridges in Lanyu pig (Sus scrofa)

T2 - Insights for human skin wound healing

AU - Lin, Chein Hong

AU - Chiu, Po Yuan

AU - Hsueh, Yuan-Yu

AU - Shieh, Shyh-Jou

AU - Wu, Chia-Ching

AU - Wong, Tak-Wah

AU - Chuong, Cheng Ming

AU - Hughes, Michael Warren

PY - 2019/4/1

Y1 - 2019/4/1

N2 - Rete ridges are important to the mechanical function of skin in animals with minimal hair, including humans. As mice do not exhibit rete ridges, the need for a quality animal model is pertinent. Here, we develop a Lanyu pig (Sus scrofa) full-thickness wound model to explore tissue regeneration because the architecture and function are similar to humans and inbred genetic variants are available. Full- and partial-thickness wounds were generated on the dorsum. Full-thickness wounds at post-wound day 57 exhibit severe scar with no signs of wound-induced hair follicle neogenesis. Wound contraction is greater in the anterior/posterior relative to the medial/lateral axis. In wound beds, K14 + cells increased while K10 + , p63 + and PCNA + cells decreased compared to unwounded tissue. Epithelial β-catenin is unchanged. The wound bed expresses more ColI, less ColIII and no elastin. Rete ridges do not form after full-thickness wounding, but incompletely regenerate after partial-thickness wounding. An alkaline phosphatase (ALP) + cell population, not associated with hair follicles, is present at the bottom of the rete ridge basal layer in pig and human unwounded skin. These K5 + /K10 − /PCNA − /ALP + epithelial cells are absent after full-thickness wounding but reappear after partial-thickness wounding, before invagination of new rete ridges. In summary, full-thickness wounding on the dorsum of Lanyu pigs results in scar formation and perturbed molecular expression while partial-thickness wounding permits limited rete ridge and papillary dermis regeneration. Future functional studies and further characterization will help contribute knowledge for the regenerative medicine field.

AB - Rete ridges are important to the mechanical function of skin in animals with minimal hair, including humans. As mice do not exhibit rete ridges, the need for a quality animal model is pertinent. Here, we develop a Lanyu pig (Sus scrofa) full-thickness wound model to explore tissue regeneration because the architecture and function are similar to humans and inbred genetic variants are available. Full- and partial-thickness wounds were generated on the dorsum. Full-thickness wounds at post-wound day 57 exhibit severe scar with no signs of wound-induced hair follicle neogenesis. Wound contraction is greater in the anterior/posterior relative to the medial/lateral axis. In wound beds, K14 + cells increased while K10 + , p63 + and PCNA + cells decreased compared to unwounded tissue. Epithelial β-catenin is unchanged. The wound bed expresses more ColI, less ColIII and no elastin. Rete ridges do not form after full-thickness wounding, but incompletely regenerate after partial-thickness wounding. An alkaline phosphatase (ALP) + cell population, not associated with hair follicles, is present at the bottom of the rete ridge basal layer in pig and human unwounded skin. These K5 + /K10 − /PCNA − /ALP + epithelial cells are absent after full-thickness wounding but reappear after partial-thickness wounding, before invagination of new rete ridges. In summary, full-thickness wounding on the dorsum of Lanyu pigs results in scar formation and perturbed molecular expression while partial-thickness wounding permits limited rete ridge and papillary dermis regeneration. Future functional studies and further characterization will help contribute knowledge for the regenerative medicine field.

UR - http://www.scopus.com/inward/record.url?scp=85062351335&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85062351335&partnerID=8YFLogxK

U2 - 10.1111/exd.13875

DO - 10.1111/exd.13875

M3 - Article

C2 - 30629757

AN - SCOPUS:85062351335

VL - 28

SP - 472

EP - 479

JO - Experimental Dermatology

JF - Experimental Dermatology

SN - 0906-6705

IS - 4

ER -