Regeneration of rete ridges in Lanyu pig (Sus scrofa): Insights for human skin wound healing

Chein Hong Lin, Po Yuan Chiu, Yuan-Yu Hsueh, Shyh-Jou Shieh, Chia-Ching Wu, Tak-Wah Wong, Cheng Ming Chuong, Michael Warren Hughes

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Abstract

Rete ridges are important to the mechanical function of skin in animals with minimal hair, including humans. As mice do not exhibit rete ridges, the need for a quality animal model is pertinent. Here, we develop a Lanyu pig (Sus scrofa) full-thickness wound model to explore tissue regeneration because the architecture and function are similar to humans and inbred genetic variants are available. Full- and partial-thickness wounds were generated on the dorsum. Full-thickness wounds at post-wound day 57 exhibit severe scar with no signs of wound-induced hair follicle neogenesis. Wound contraction is greater in the anterior/posterior relative to the medial/lateral axis. In wound beds, K14 + cells increased while K10 + , p63 + and PCNA + cells decreased compared to unwounded tissue. Epithelial β-catenin is unchanged. The wound bed expresses more ColI, less ColIII and no elastin. Rete ridges do not form after full-thickness wounding, but incompletely regenerate after partial-thickness wounding. An alkaline phosphatase (ALP) + cell population, not associated with hair follicles, is present at the bottom of the rete ridge basal layer in pig and human unwounded skin. These K5 + /K10 /PCNA /ALP + epithelial cells are absent after full-thickness wounding but reappear after partial-thickness wounding, before invagination of new rete ridges. In summary, full-thickness wounding on the dorsum of Lanyu pigs results in scar formation and perturbed molecular expression while partial-thickness wounding permits limited rete ridge and papillary dermis regeneration. Future functional studies and further characterization will help contribute knowledge for the regenerative medicine field.

Original languageEnglish
Pages (from-to)472-479
Number of pages8
JournalExperimental Dermatology
Volume28
Issue number4
DOIs
Publication statusPublished - 2019 Apr 1

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All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Dermatology

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