Regulation of plastid rDNA transcription by interaction of CDF2 with two different RNA polymerases

Muriel Bligny, Florence Courtois, Safia Thaminy, Ching-Chun Chang, Thierry Lagrange, Jahnabi Baruah-Wolff, David Stern, Silva Lerbs-Mache

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

The plastid genome is known to be transcribed by a plastid-encoded prokaryotic-type RNA polymerase (PEP) and by a nucleus-encoded phage-type RNA polymerase (NEP). The spinach plastid rrn operon promoter region harbours three different, overlapping promoters. Two of them are of the prokaryotic type. The third promoter is a non-consensus-type NEP promoter. We separated three different transcriptional activities from spinach chloroplasts: PEP, the phage-type RNA polymerase NEP-1, and a third, hitherto undescribed transcriptional activity (NEP-2). NEP-2 specifically transcribes the rrn operon in the presence of the transcription factor CDF2. CDF2 was previously shown to recruit PEP to the rrn promoter to repress transcription. Together, our results suggest the existence of a third RNA polymerase in plastids and a mechanism of rDNA transcriptional regulation that is based on the interaction of the transcription factor CDF2 with two different transcriptional systems.

Original languageEnglish
Pages (from-to)1851-1860
Number of pages10
JournalEMBO Journal
Volume19
Issue number8
Publication statusPublished - 2000 Apr 17

Fingerprint

Plastids
DNA-Directed RNA Polymerases
Transcription
Ribosomal DNA
Spinacia oleracea
Operon
Transcription Factors
Plastid Genomes
Bacteriophages
Chloroplasts
Ports and harbors
Genetic Promoter Regions
Genes
Peptamen

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Bligny, M., Courtois, F., Thaminy, S., Chang, C-C., Lagrange, T., Baruah-Wolff, J., ... Lerbs-Mache, S. (2000). Regulation of plastid rDNA transcription by interaction of CDF2 with two different RNA polymerases. EMBO Journal, 19(8), 1851-1860.
Bligny, Muriel ; Courtois, Florence ; Thaminy, Safia ; Chang, Ching-Chun ; Lagrange, Thierry ; Baruah-Wolff, Jahnabi ; Stern, David ; Lerbs-Mache, Silva. / Regulation of plastid rDNA transcription by interaction of CDF2 with two different RNA polymerases. In: EMBO Journal. 2000 ; Vol. 19, No. 8. pp. 1851-1860.
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Bligny, M, Courtois, F, Thaminy, S, Chang, C-C, Lagrange, T, Baruah-Wolff, J, Stern, D & Lerbs-Mache, S 2000, 'Regulation of plastid rDNA transcription by interaction of CDF2 with two different RNA polymerases', EMBO Journal, vol. 19, no. 8, pp. 1851-1860.

Regulation of plastid rDNA transcription by interaction of CDF2 with two different RNA polymerases. / Bligny, Muriel; Courtois, Florence; Thaminy, Safia; Chang, Ching-Chun; Lagrange, Thierry; Baruah-Wolff, Jahnabi; Stern, David; Lerbs-Mache, Silva.

In: EMBO Journal, Vol. 19, No. 8, 17.04.2000, p. 1851-1860.

Research output: Contribution to journalArticle

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AU - Baruah-Wolff, Jahnabi

AU - Stern, David

AU - Lerbs-Mache, Silva

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Bligny M, Courtois F, Thaminy S, Chang C-C, Lagrange T, Baruah-Wolff J et al. Regulation of plastid rDNA transcription by interaction of CDF2 with two different RNA polymerases. EMBO Journal. 2000 Apr 17;19(8):1851-1860.