Regulation of prostaglandin F(2α) and E receptor mRNA by prostaglandin F(2α) in ovine corpora lutea

Shaw-Jenq Tsai, L. E. Anderson, J. Juengel, G. D. Niswender, M. C. Wiltbank

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Prostaglandins regulate many physiological functions, including reproduction, by binding to specific plasma membrane receptors. In this study we evaluated the regulation of PGF(2α) (FP) and PGE (EP3 subtype) receptors in ovine corpora lutea. In the first study, tissue distribution of FP and EP3 receptors was evaluated in 13 ovine tissues. FP receptor mRNA was present in 100-fold higher concentration in corpora lutea than in other tissues. Similarly, [3H]PGF(2α) binding was much greater in luteal plasma membranes than in membranes from other tissues. In contrast, EP3 receptor mRNA was more uniformly distributed, with high concentrations in adrenal medulla, inner myometrium, kidney medulla and heart. The distribution of [3H]PGE1 binding was generally similar to EP3 mRNA, with the exception that ovarian stroma, endometrium and outer myometrium had high [3H]PGE1 binding but low concentrations of EP3 receptor mRNA. The second study evaluated the action of PGF(2α) on luteal mRNA encoding FP and EP3 receptors. Ewes had PGF(2α) or saline infused into the ovarian artery and corpora lutea were removed at 0, 1, 4, 12 and 24 h. FP receptor mRNA decreased by 50% at 12 and 24 h after infusion with PGF(2α), whereas EP3 mRNA was unchanged. Treatment of large luteal cells with PGF(2α), phorbol didecanoate (protein kinase C activator), or ionomycin (calcium ionophore) decreased FP receptor mRNA after 24 h (P < 0.05). Glyceraldehyde 3-phosphate dehydrogenase mRNA was not changed by any treatment. These results show that EP3 receptors are expressed in many tissues and expression is not regulated by PGF(2α). In contrast, FP receptors are primarily expressed in corpora lutea and expression is inhibited by PGF(2α).

Original languageEnglish
Pages (from-to)69-75
Number of pages7
JournalJournal of Reproduction and Fertility
Volume114
Issue number1
Publication statusPublished - 1998 Dec 5

Fingerprint

Corpus Luteum
Prostaglandins F
Prostaglandins E
Sheep
Messenger RNA
Myometrium
Alprostadil
Receptors, Prostaglandin E, EP3 Subtype
Cell Membrane
Kidney Medulla
Luteal Cells
Ionomycin
Glyceraldehyde-3-Phosphate Dehydrogenases
Adrenal Medulla
Calcium Ionophores
Tissue Distribution
Endometrium
Protein Kinase C
Prostaglandins
Reproduction

All Science Journal Classification (ASJC) codes

  • Physiology
  • Embryology
  • Molecular Biology
  • Obstetrics and Gynaecology
  • Developmental Biology

Cite this

Tsai, Shaw-Jenq ; Anderson, L. E. ; Juengel, J. ; Niswender, G. D. ; Wiltbank, M. C. / Regulation of prostaglandin F(2α) and E receptor mRNA by prostaglandin F(2α) in ovine corpora lutea. In: Journal of Reproduction and Fertility. 1998 ; Vol. 114, No. 1. pp. 69-75.
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Regulation of prostaglandin F(2α) and E receptor mRNA by prostaglandin F(2α) in ovine corpora lutea. / Tsai, Shaw-Jenq; Anderson, L. E.; Juengel, J.; Niswender, G. D.; Wiltbank, M. C.

In: Journal of Reproduction and Fertility, Vol. 114, No. 1, 05.12.1998, p. 69-75.

Research output: Contribution to journalArticle

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