Regulatory mechanism of Cordyceps sinensis mycelium on mouse Leydig cell steroidogenesis

Chih Chao Hsu, Shaw Jeng Tsai, Yuan Li Huang, Bu Miin Huang

Research output: Contribution to journalArticlepeer-review

38 Citations (Scopus)

Abstract

We demonstrate the mechanism by which Cordyceps sinensis (CS) mycelium regulates Leydig cell steroidogenesis. Mouse Leydig cells were treated with forskolin, H89, phorbol 12-myristate 13-acetate, staurosporine, or steroidogenic enzyme precursors with or without 3 mg/ml CS; then testosterone production was determined. H89, but not phorbol 12-myristate 13-acetate or staurosporine, decreased CS-treated Leydig cell steroidogenesis. CS inhibited Leydig cell steroidogenesis by suppressing the activity of P450scc enzyme, but not 3β-hydroxysteroid dehydrogenase, 17α-hydroxylase, 20α-hydroxylase, or 17β-hydroxysteroid dehydrogenase enzymes. Thus, CS activated the cAMP-protein kinase A signal pathway, but not protein kinase C, and attenuated P45scc enzyme activity to reduce human chorionic gonadotropin-stimulated steroidogenesis in purified mouse Leydig cells.

Original languageEnglish
Pages (from-to)140-143
Number of pages4
JournalFEBS Letters
Volume543
Issue number1-3
DOIs
Publication statusPublished - 2003 May 22

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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