TY - JOUR
T1 - Regulatory mechanism of Cordyceps sinensis mycelium on mouse Leydig cell steroidogenesis
AU - Hsu, Chih Chao
AU - Tsai, Shaw Jeng
AU - Huang, Yuan Li
AU - Huang, Bu Miin
N1 - Funding Information:
This study was supported by NSC 91-2320-B-006-070 from the National Science Council of the Republic of China to B.M.H.
PY - 2003/5/22
Y1 - 2003/5/22
N2 - We demonstrate the mechanism by which Cordyceps sinensis (CS) mycelium regulates Leydig cell steroidogenesis. Mouse Leydig cells were treated with forskolin, H89, phorbol 12-myristate 13-acetate, staurosporine, or steroidogenic enzyme precursors with or without 3 mg/ml CS; then testosterone production was determined. H89, but not phorbol 12-myristate 13-acetate or staurosporine, decreased CS-treated Leydig cell steroidogenesis. CS inhibited Leydig cell steroidogenesis by suppressing the activity of P450scc enzyme, but not 3β-hydroxysteroid dehydrogenase, 17α-hydroxylase, 20α-hydroxylase, or 17β-hydroxysteroid dehydrogenase enzymes. Thus, CS activated the cAMP-protein kinase A signal pathway, but not protein kinase C, and attenuated P45scc enzyme activity to reduce human chorionic gonadotropin-stimulated steroidogenesis in purified mouse Leydig cells.
AB - We demonstrate the mechanism by which Cordyceps sinensis (CS) mycelium regulates Leydig cell steroidogenesis. Mouse Leydig cells were treated with forskolin, H89, phorbol 12-myristate 13-acetate, staurosporine, or steroidogenic enzyme precursors with or without 3 mg/ml CS; then testosterone production was determined. H89, but not phorbol 12-myristate 13-acetate or staurosporine, decreased CS-treated Leydig cell steroidogenesis. CS inhibited Leydig cell steroidogenesis by suppressing the activity of P450scc enzyme, but not 3β-hydroxysteroid dehydrogenase, 17α-hydroxylase, 20α-hydroxylase, or 17β-hydroxysteroid dehydrogenase enzymes. Thus, CS activated the cAMP-protein kinase A signal pathway, but not protein kinase C, and attenuated P45scc enzyme activity to reduce human chorionic gonadotropin-stimulated steroidogenesis in purified mouse Leydig cells.
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U2 - 10.1016/S0014-5793(03)00427-7
DO - 10.1016/S0014-5793(03)00427-7
M3 - Article
C2 - 12753921
AN - SCOPUS:0037716937
SN - 0014-5793
VL - 543
SP - 140
EP - 143
JO - FEBS Letters
JF - FEBS Letters
IS - 1-3
ER -