TY - JOUR
T1 - Rejuvenation in vitro
T2 - Modulation of Protein Phosphorylation in Sequoia sempervirens
AU - Kuo, Jung lieh
AU - Huang, Hao jen
AU - Cheng, Ching ming
AU - Chen, Li jing
AU - Huang, Bau lian
AU - Huang, Li chun
AU - Kuo, Tsong teh
N1 - Funding Information:
We are most grateful to Professor Toshio Murashige for critical reading of the manuscript. This work was funded by the National Science Council of the Republic of China.
PY - 1995
Y1 - 1995
N2 - Crude extract of whole cells and isolated mitochondria from cultured Sequoia sempervirens shoots of the adult, juvenile, and grafted adult tissues were incubated with [γ-32P]-ATP, and the resultant phosphoproteins were then separated by SDS-PAGE and examined through autoradiography. In the extracts of whole cells, the phosphorylation of their 32-kDa protein was evident only in the adult, and that of the 31-kDa protein was detected only in the juvenile cells. Repeated graftings of shoots from adult trees onto juvenile rootstock results in changing the phosphoprotein pattern. It becomes like that of the juvenile tissue and becomes very similar after four grafts. However, no qualitative differences were noted in mitochondrial phosphorylation patterns; rather, the total 32P incorporation turned out to be lower in the adult tissue than in juvenile and rejuvenated tissues.
AB - Crude extract of whole cells and isolated mitochondria from cultured Sequoia sempervirens shoots of the adult, juvenile, and grafted adult tissues were incubated with [γ-32P]-ATP, and the resultant phosphoproteins were then separated by SDS-PAGE and examined through autoradiography. In the extracts of whole cells, the phosphorylation of their 32-kDa protein was evident only in the adult, and that of the 31-kDa protein was detected only in the juvenile cells. Repeated graftings of shoots from adult trees onto juvenile rootstock results in changing the phosphoprotein pattern. It becomes like that of the juvenile tissue and becomes very similar after four grafts. However, no qualitative differences were noted in mitochondrial phosphorylation patterns; rather, the total 32P incorporation turned out to be lower in the adult tissue than in juvenile and rejuvenated tissues.
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U2 - 10.1016/S0176-1617(11)82064-X
DO - 10.1016/S0176-1617(11)82064-X
M3 - Article
AN - SCOPUS:0028851071
SN - 0176-1617
VL - 146
SP - 333
EP - 336
JO - Journal of Plant Physiology
JF - Journal of Plant Physiology
IS - 3
ER -