Rejuvenation of Sequoia sempervirens in vitro: Changes in isoesterases and isoperoxidases

Hao Jen Huang, Yu Chen, Jung Lieh Kuo, Tsong Teh Kuo, Chai Chong Tzeng, Bau Lian Huang, Chung Mong Chen, Li Chun Huang

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Abstract

Rejuvenation, as evidenced by restored rooting competence, of Sequoia sempervirens by a series of repeated grafting of adult shoot tips onto juvenile rootstocks in vitro was confirmed. Complete restoration of rooting competence was achieved after grafting repeatedly five times. The rejuvenation was correlated with a disappearance of adult-associated esterase and peroxidase isozymes and an appearance of isoesterases and isoperoxidases that were characteristic of juveuile-phase shoots, These esterase and peroxidase isozymes could serve as markers to assist phasechange investigations.

Original languageEnglish
Pages (from-to)77-80
Number of pages4
JournalPlant and Cell Physiology
Volume37
Issue number1
DOIs
Publication statusPublished - 1996 Jan

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science
  • Cell Biology

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    Huang, H. J., Chen, Y., Kuo, J. L., Kuo, T. T., Tzeng, C. C., Huang, B. L., Chen, C. M., & Huang, L. C. (1996). Rejuvenation of Sequoia sempervirens in vitro: Changes in isoesterases and isoperoxidases. Plant and Cell Physiology, 37(1), 77-80. https://doi.org/10.1093/oxfordjournals.pcp.a028916