Relationship between fibroblast growth factor 23 and biochemical and bone histomorphometric alterations in a chronic kidney disease rat model undergoing parathyroidectomy

Hung Wei Liao, Peir Haur Hung, Chih Yen Hsiao, Hung Hsiang Liou, Hsin Shih Lin, Tsang-Hai Huang, I. Ming Jou, Kuen-Jer Tsai

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: Phosphate burden in chronic kidney disease (CKD) leads to elevated serum fibroblast factor- 23 (FGF-23) levels, secondary hyperparathyroidism and chronic kidney disease-mineral bone disorder (CKD-MBD). However dissociated hyperphosphatemia and low serum FGF- 23 concentrations have been observed in experimentally parathyoridectomized rats. The relationships between serum mineral, hormone, and bone metabolism may be altered in the presence of CKD. The aim of our study was to investigate whether a consistent relationship existed between serum FGF-23 levels, specific serum biochemical markers, and histomorphometric parameters of bone metabolism in a parathyroidectomized CKD animal model. Results: Sprague Dawley rats were divided into 3 groups: parathyroidectomy (PTX) and CKD (PTX +CKD, 9 rats), CKD without PTX (CKD, 9 rats), and neither PTX nor CKD (sham-operated control, 8 rats); CKD was induced by partial nephrectomy. At 8 weeks after partial nephrectomy, serum biomarkers were measured. Bone histomorphometries of the distal femoral metaphyseal bone were analyzed. The mean serum FGF-23 levels and mean bone formation rate were the highest in the CKD group and the lowest in the PTX+CKD group. Bone volume parameters increased significantly in the PTX+CKD group. Pearson's correlation revealed that serum FGF-23 levels associated with those of intact parathyroid hormone, phosphate, collagen type I C-telopeptide, and calcium. Univariate linear regression showed that serum FGF-23 values correlated with bone formation rate, bone volume, and osteoid parameters. Stepwise multivariate regression analysis revealed that circulating FGF-23 values were independently associated with bone volume and thickness (β = -0.737; p < 0.001 and β = -0.526; p = 0.006, respectively). Serum parathyroid hormone levels independently correlated with bone formation rate (β = 0.714; p < 0.001) while collagen type I C-telopeptide levels correlated with osteoid parameter. Conclusion: Serum FGF-23 levels independently correlated with bone volume parameters in rats with experimentally induced CKD.

Original languageEnglish
Article numbere0133278
JournalPloS one
Volume10
Issue number7
DOIs
Publication statusPublished - 2015 Jul 17

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fibroblast growth factors
Parathyroidectomy
disease models
kidney diseases
Chronic Renal Insufficiency
Rats
Bone
animal models
bones
Bone and Bones
Serum
rats
Osteogenesis
bone formation
bone metabolism
Biomarkers
parathyroid hormone
Collagen Type I
Nephrectomy
Parathyroid Hormone

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

@article{f99aaf20608c4f3ebadec54aa67489b7,
title = "Relationship between fibroblast growth factor 23 and biochemical and bone histomorphometric alterations in a chronic kidney disease rat model undergoing parathyroidectomy",
abstract = "Background: Phosphate burden in chronic kidney disease (CKD) leads to elevated serum fibroblast factor- 23 (FGF-23) levels, secondary hyperparathyroidism and chronic kidney disease-mineral bone disorder (CKD-MBD). However dissociated hyperphosphatemia and low serum FGF- 23 concentrations have been observed in experimentally parathyoridectomized rats. The relationships between serum mineral, hormone, and bone metabolism may be altered in the presence of CKD. The aim of our study was to investigate whether a consistent relationship existed between serum FGF-23 levels, specific serum biochemical markers, and histomorphometric parameters of bone metabolism in a parathyroidectomized CKD animal model. Results: Sprague Dawley rats were divided into 3 groups: parathyroidectomy (PTX) and CKD (PTX +CKD, 9 rats), CKD without PTX (CKD, 9 rats), and neither PTX nor CKD (sham-operated control, 8 rats); CKD was induced by partial nephrectomy. At 8 weeks after partial nephrectomy, serum biomarkers were measured. Bone histomorphometries of the distal femoral metaphyseal bone were analyzed. The mean serum FGF-23 levels and mean bone formation rate were the highest in the CKD group and the lowest in the PTX+CKD group. Bone volume parameters increased significantly in the PTX+CKD group. Pearson's correlation revealed that serum FGF-23 levels associated with those of intact parathyroid hormone, phosphate, collagen type I C-telopeptide, and calcium. Univariate linear regression showed that serum FGF-23 values correlated with bone formation rate, bone volume, and osteoid parameters. Stepwise multivariate regression analysis revealed that circulating FGF-23 values were independently associated with bone volume and thickness (β = -0.737; p < 0.001 and β = -0.526; p = 0.006, respectively). Serum parathyroid hormone levels independently correlated with bone formation rate (β = 0.714; p < 0.001) while collagen type I C-telopeptide levels correlated with osteoid parameter. Conclusion: Serum FGF-23 levels independently correlated with bone volume parameters in rats with experimentally induced CKD.",
author = "Liao, {Hung Wei} and Hung, {Peir Haur} and Hsiao, {Chih Yen} and Liou, {Hung Hsiang} and Lin, {Hsin Shih} and Tsang-Hai Huang and Jou, {I. Ming} and Kuen-Jer Tsai",
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Relationship between fibroblast growth factor 23 and biochemical and bone histomorphometric alterations in a chronic kidney disease rat model undergoing parathyroidectomy. / Liao, Hung Wei; Hung, Peir Haur; Hsiao, Chih Yen; Liou, Hung Hsiang; Lin, Hsin Shih; Huang, Tsang-Hai; Jou, I. Ming; Tsai, Kuen-Jer.

In: PloS one, Vol. 10, No. 7, e0133278, 17.07.2015.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Relationship between fibroblast growth factor 23 and biochemical and bone histomorphometric alterations in a chronic kidney disease rat model undergoing parathyroidectomy

AU - Liao, Hung Wei

AU - Hung, Peir Haur

AU - Hsiao, Chih Yen

AU - Liou, Hung Hsiang

AU - Lin, Hsin Shih

AU - Huang, Tsang-Hai

AU - Jou, I. Ming

AU - Tsai, Kuen-Jer

PY - 2015/7/17

Y1 - 2015/7/17

N2 - Background: Phosphate burden in chronic kidney disease (CKD) leads to elevated serum fibroblast factor- 23 (FGF-23) levels, secondary hyperparathyroidism and chronic kidney disease-mineral bone disorder (CKD-MBD). However dissociated hyperphosphatemia and low serum FGF- 23 concentrations have been observed in experimentally parathyoridectomized rats. The relationships between serum mineral, hormone, and bone metabolism may be altered in the presence of CKD. The aim of our study was to investigate whether a consistent relationship existed between serum FGF-23 levels, specific serum biochemical markers, and histomorphometric parameters of bone metabolism in a parathyroidectomized CKD animal model. Results: Sprague Dawley rats were divided into 3 groups: parathyroidectomy (PTX) and CKD (PTX +CKD, 9 rats), CKD without PTX (CKD, 9 rats), and neither PTX nor CKD (sham-operated control, 8 rats); CKD was induced by partial nephrectomy. At 8 weeks after partial nephrectomy, serum biomarkers were measured. Bone histomorphometries of the distal femoral metaphyseal bone were analyzed. The mean serum FGF-23 levels and mean bone formation rate were the highest in the CKD group and the lowest in the PTX+CKD group. Bone volume parameters increased significantly in the PTX+CKD group. Pearson's correlation revealed that serum FGF-23 levels associated with those of intact parathyroid hormone, phosphate, collagen type I C-telopeptide, and calcium. Univariate linear regression showed that serum FGF-23 values correlated with bone formation rate, bone volume, and osteoid parameters. Stepwise multivariate regression analysis revealed that circulating FGF-23 values were independently associated with bone volume and thickness (β = -0.737; p < 0.001 and β = -0.526; p = 0.006, respectively). Serum parathyroid hormone levels independently correlated with bone formation rate (β = 0.714; p < 0.001) while collagen type I C-telopeptide levels correlated with osteoid parameter. Conclusion: Serum FGF-23 levels independently correlated with bone volume parameters in rats with experimentally induced CKD.

AB - Background: Phosphate burden in chronic kidney disease (CKD) leads to elevated serum fibroblast factor- 23 (FGF-23) levels, secondary hyperparathyroidism and chronic kidney disease-mineral bone disorder (CKD-MBD). However dissociated hyperphosphatemia and low serum FGF- 23 concentrations have been observed in experimentally parathyoridectomized rats. The relationships between serum mineral, hormone, and bone metabolism may be altered in the presence of CKD. The aim of our study was to investigate whether a consistent relationship existed between serum FGF-23 levels, specific serum biochemical markers, and histomorphometric parameters of bone metabolism in a parathyroidectomized CKD animal model. Results: Sprague Dawley rats were divided into 3 groups: parathyroidectomy (PTX) and CKD (PTX +CKD, 9 rats), CKD without PTX (CKD, 9 rats), and neither PTX nor CKD (sham-operated control, 8 rats); CKD was induced by partial nephrectomy. At 8 weeks after partial nephrectomy, serum biomarkers were measured. Bone histomorphometries of the distal femoral metaphyseal bone were analyzed. The mean serum FGF-23 levels and mean bone formation rate were the highest in the CKD group and the lowest in the PTX+CKD group. Bone volume parameters increased significantly in the PTX+CKD group. Pearson's correlation revealed that serum FGF-23 levels associated with those of intact parathyroid hormone, phosphate, collagen type I C-telopeptide, and calcium. Univariate linear regression showed that serum FGF-23 values correlated with bone formation rate, bone volume, and osteoid parameters. Stepwise multivariate regression analysis revealed that circulating FGF-23 values were independently associated with bone volume and thickness (β = -0.737; p < 0.001 and β = -0.526; p = 0.006, respectively). Serum parathyroid hormone levels independently correlated with bone formation rate (β = 0.714; p < 0.001) while collagen type I C-telopeptide levels correlated with osteoid parameter. Conclusion: Serum FGF-23 levels independently correlated with bone volume parameters in rats with experimentally induced CKD.

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