Repeated intratracheal inoculation of house dust mite (Dermatophagoides farinae) induces pulmonary eosinophilic inflammation and IgE antibody production in mice

Chun Keung Yu, Cher Ming Shieh, Huan Yao Lei

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

Background: In vitro studies have shown that the biologic activities of dust mite allergens probably contribute to their allergenicity. However, little is known about their in vivo effect, which may lead to allergic inflammation and sensitization. Objective: In this study we characterized the pathologic and immunologic responses of mice after repetitive challenge with dust mite Dermatophagoides farinae. Methods: Der f crude extract was intratracheally instilled into female BALB/c mice either once or a total of 10 times at 1-week intervals. The inflammatory responses, morphologic changes, IgE antibody and cytokine levels, and costimulatory molecular B7 expression in the airways were then monitored. Results: We demonstrated that single Der f challenge provoked an acute neutrophilic inflammation in the airways. After repeated Der f challenge, there was chronic inflammation characterized by increased numbers of lymphocytes and eosinophils in bronchoalveolar lavage (BAL) fluids. Histopathologically, the numbers of goblet cells and mast cells were significantly increased in the airways of these mice. Repetitive challenge elicited Der f-specific IgE antibody, increased IL-5 and IFN-γ level in BAL fluids, and enhanced costimulatory B7 molecule expression on BAL cells. Conclusions: Our findings are in agreement with those of other in vitro studies concerning the properties of dust mite allergens. Prolonged inhalation of Der f without adjuvant may represent an optimal condition to develop experimental animal models of allergic lung diseases.

Original languageEnglish
Pages (from-to)228-236
Number of pages9
JournalJournal of Allergy and Clinical Immunology
Volume104
Issue number1
DOIs
Publication statusPublished - 1999 Jan 1

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'Repeated intratracheal inoculation of house dust mite (Dermatophagoides farinae) induces pulmonary eosinophilic inflammation and IgE antibody production in mice'. Together they form a unique fingerprint.

Cite this