TY - JOUR
T1 - Repeated intratracheal inoculation of house dust mite (Dermatophagoides farinae) induces pulmonary eosinophilic inflammation and IgE antibody production in mice
AU - Yu, Chun Keung
AU - Shieh, Cher Ming
AU - Lei, Huan Yao
N1 - Funding Information:
Supported by grant NSC87-2314-B006-085 from the National Science Council, Republic of China.
PY - 1999
Y1 - 1999
N2 - Background: In vitro studies have shown that the biologic activities of dust mite allergens probably contribute to their allergenicity. However, little is known about their in vivo effect, which may lead to allergic inflammation and sensitization. Objective: In this study we characterized the pathologic and immunologic responses of mice after repetitive challenge with dust mite Dermatophagoides farinae. Methods: Der f crude extract was intratracheally instilled into female BALB/c mice either once or a total of 10 times at 1-week intervals. The inflammatory responses, morphologic changes, IgE antibody and cytokine levels, and costimulatory molecular B7 expression in the airways were then monitored. Results: We demonstrated that single Der f challenge provoked an acute neutrophilic inflammation in the airways. After repeated Der f challenge, there was chronic inflammation characterized by increased numbers of lymphocytes and eosinophils in bronchoalveolar lavage (BAL) fluids. Histopathologically, the numbers of goblet cells and mast cells were significantly increased in the airways of these mice. Repetitive challenge elicited Der f-specific IgE antibody, increased IL-5 and IFN-γ level in BAL fluids, and enhanced costimulatory B7 molecule expression on BAL cells. Conclusions: Our findings are in agreement with those of other in vitro studies concerning the properties of dust mite allergens. Prolonged inhalation of Der f without adjuvant may represent an optimal condition to develop experimental animal models of allergic lung diseases.
AB - Background: In vitro studies have shown that the biologic activities of dust mite allergens probably contribute to their allergenicity. However, little is known about their in vivo effect, which may lead to allergic inflammation and sensitization. Objective: In this study we characterized the pathologic and immunologic responses of mice after repetitive challenge with dust mite Dermatophagoides farinae. Methods: Der f crude extract was intratracheally instilled into female BALB/c mice either once or a total of 10 times at 1-week intervals. The inflammatory responses, morphologic changes, IgE antibody and cytokine levels, and costimulatory molecular B7 expression in the airways were then monitored. Results: We demonstrated that single Der f challenge provoked an acute neutrophilic inflammation in the airways. After repeated Der f challenge, there was chronic inflammation characterized by increased numbers of lymphocytes and eosinophils in bronchoalveolar lavage (BAL) fluids. Histopathologically, the numbers of goblet cells and mast cells were significantly increased in the airways of these mice. Repetitive challenge elicited Der f-specific IgE antibody, increased IL-5 and IFN-γ level in BAL fluids, and enhanced costimulatory B7 molecule expression on BAL cells. Conclusions: Our findings are in agreement with those of other in vitro studies concerning the properties of dust mite allergens. Prolonged inhalation of Der f without adjuvant may represent an optimal condition to develop experimental animal models of allergic lung diseases.
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U2 - 10.1016/S0091-6749(99)70140-5
DO - 10.1016/S0091-6749(99)70140-5
M3 - Article
C2 - 10400866
AN - SCOPUS:0032865503
SN - 0091-6749
VL - 104
SP - 228
EP - 236
JO - Journal of Allergy and Clinical Immunology
JF - Journal of Allergy and Clinical Immunology
IS - 1
ER -