Residue depletion study of danofloxacin in cultured tilapia (Oreochromis mossambicus)

Yang Chi Fan, Shi Yuan Sheu, Hong Thih Lai, Ming Huang Chang, Pei Heng Chen, Yi Chih Lei, Tzong Fu Kuo, Chia-Yih Wang

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Danofloxacin is an antibacterial drug of the fluoroquinolone group developed for therapeutic purposes in veterinary medicine. The studies described here include investigations of the residues following a single dose or multiple doses of danofloxacin. Residue depletion studies were performed to determine residues in plasma and tissues of saltwater tilapia fish (Oreochromis mossambicus) after a single oral administration of danofloxacin at the dose of 10 mg/kg body weight and also after daily dose of 10 mg/kg body weight for five consecutive days. Danofloxacin residues were analyzed by HPLC with fluorescence detection. Following a single oral dose, danofloxacin residues in 6 h postdosing tilapia were at a maximum of 1.44, 12.48, and 13.18 μg/g in serum, liver, and kidney samples, respectively, while a peak muscle concentration of 2.15 μg/g was reached at 12 h. From single-dose data, the concentration of danofloxacin in serum, muscle, liver, and kidney samples declined with half-lives of 29, 34, 49, and 44 h, respectively. Based on the maximum residue level (MRL) of 0.1 μg/g in edible tissue for fin fish, the withdrawal times of danofloxacin in muscle were estimated to fall below the MRL after a withdrawal period of 21 days following the multipledose administration. These results may be helpful to regulatory agencies as they determine what tissues should be monitored to ensure that the established residue safety tolerance levels are not exceeded.

Original languageEnglish
Pages (from-to)575-579
Number of pages5
JournalJournal of AOAC International
Volume98
Issue number3
DOIs
Publication statusPublished - 2015 May 1

Fingerprint

danofloxacin
Tilapia (Cichlidae)
Tilapia
Oreochromis mossambicus
dosage
muscle
Muscle
maximum residue limits
Tissue
serum
Muscles
Liver
muscles
Fish
Fishes
Veterinary medicine
Body Weight
kidneys
Kidney
liver

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Food Science
  • Environmental Chemistry
  • Agronomy and Crop Science
  • Pharmacology

Cite this

Fan, Yang Chi ; Sheu, Shi Yuan ; Lai, Hong Thih ; Chang, Ming Huang ; Chen, Pei Heng ; Lei, Yi Chih ; Kuo, Tzong Fu ; Wang, Chia-Yih. / Residue depletion study of danofloxacin in cultured tilapia (Oreochromis mossambicus). In: Journal of AOAC International. 2015 ; Vol. 98, No. 3. pp. 575-579.
@article{850836e596984a39aeb601105f629fdd,
title = "Residue depletion study of danofloxacin in cultured tilapia (Oreochromis mossambicus)",
abstract = "Danofloxacin is an antibacterial drug of the fluoroquinolone group developed for therapeutic purposes in veterinary medicine. The studies described here include investigations of the residues following a single dose or multiple doses of danofloxacin. Residue depletion studies were performed to determine residues in plasma and tissues of saltwater tilapia fish (Oreochromis mossambicus) after a single oral administration of danofloxacin at the dose of 10 mg/kg body weight and also after daily dose of 10 mg/kg body weight for five consecutive days. Danofloxacin residues were analyzed by HPLC with fluorescence detection. Following a single oral dose, danofloxacin residues in 6 h postdosing tilapia were at a maximum of 1.44, 12.48, and 13.18 μg/g in serum, liver, and kidney samples, respectively, while a peak muscle concentration of 2.15 μg/g was reached at 12 h. From single-dose data, the concentration of danofloxacin in serum, muscle, liver, and kidney samples declined with half-lives of 29, 34, 49, and 44 h, respectively. Based on the maximum residue level (MRL) of 0.1 μg/g in edible tissue for fin fish, the withdrawal times of danofloxacin in muscle were estimated to fall below the MRL after a withdrawal period of 21 days following the multipledose administration. These results may be helpful to regulatory agencies as they determine what tissues should be monitored to ensure that the established residue safety tolerance levels are not exceeded.",
author = "Fan, {Yang Chi} and Sheu, {Shi Yuan} and Lai, {Hong Thih} and Chang, {Ming Huang} and Chen, {Pei Heng} and Lei, {Yi Chih} and Kuo, {Tzong Fu} and Chia-Yih Wang",
year = "2015",
month = "5",
day = "1",
doi = "10.5740/jaoacint.13-327",
language = "English",
volume = "98",
pages = "575--579",
journal = "Journal of AOAC International",
issn = "1060-3271",
publisher = "AOAC International",
number = "3",

}

Residue depletion study of danofloxacin in cultured tilapia (Oreochromis mossambicus). / Fan, Yang Chi; Sheu, Shi Yuan; Lai, Hong Thih; Chang, Ming Huang; Chen, Pei Heng; Lei, Yi Chih; Kuo, Tzong Fu; Wang, Chia-Yih.

In: Journal of AOAC International, Vol. 98, No. 3, 01.05.2015, p. 575-579.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Residue depletion study of danofloxacin in cultured tilapia (Oreochromis mossambicus)

AU - Fan, Yang Chi

AU - Sheu, Shi Yuan

AU - Lai, Hong Thih

AU - Chang, Ming Huang

AU - Chen, Pei Heng

AU - Lei, Yi Chih

AU - Kuo, Tzong Fu

AU - Wang, Chia-Yih

PY - 2015/5/1

Y1 - 2015/5/1

N2 - Danofloxacin is an antibacterial drug of the fluoroquinolone group developed for therapeutic purposes in veterinary medicine. The studies described here include investigations of the residues following a single dose or multiple doses of danofloxacin. Residue depletion studies were performed to determine residues in plasma and tissues of saltwater tilapia fish (Oreochromis mossambicus) after a single oral administration of danofloxacin at the dose of 10 mg/kg body weight and also after daily dose of 10 mg/kg body weight for five consecutive days. Danofloxacin residues were analyzed by HPLC with fluorescence detection. Following a single oral dose, danofloxacin residues in 6 h postdosing tilapia were at a maximum of 1.44, 12.48, and 13.18 μg/g in serum, liver, and kidney samples, respectively, while a peak muscle concentration of 2.15 μg/g was reached at 12 h. From single-dose data, the concentration of danofloxacin in serum, muscle, liver, and kidney samples declined with half-lives of 29, 34, 49, and 44 h, respectively. Based on the maximum residue level (MRL) of 0.1 μg/g in edible tissue for fin fish, the withdrawal times of danofloxacin in muscle were estimated to fall below the MRL after a withdrawal period of 21 days following the multipledose administration. These results may be helpful to regulatory agencies as they determine what tissues should be monitored to ensure that the established residue safety tolerance levels are not exceeded.

AB - Danofloxacin is an antibacterial drug of the fluoroquinolone group developed for therapeutic purposes in veterinary medicine. The studies described here include investigations of the residues following a single dose or multiple doses of danofloxacin. Residue depletion studies were performed to determine residues in plasma and tissues of saltwater tilapia fish (Oreochromis mossambicus) after a single oral administration of danofloxacin at the dose of 10 mg/kg body weight and also after daily dose of 10 mg/kg body weight for five consecutive days. Danofloxacin residues were analyzed by HPLC with fluorescence detection. Following a single oral dose, danofloxacin residues in 6 h postdosing tilapia were at a maximum of 1.44, 12.48, and 13.18 μg/g in serum, liver, and kidney samples, respectively, while a peak muscle concentration of 2.15 μg/g was reached at 12 h. From single-dose data, the concentration of danofloxacin in serum, muscle, liver, and kidney samples declined with half-lives of 29, 34, 49, and 44 h, respectively. Based on the maximum residue level (MRL) of 0.1 μg/g in edible tissue for fin fish, the withdrawal times of danofloxacin in muscle were estimated to fall below the MRL after a withdrawal period of 21 days following the multipledose administration. These results may be helpful to regulatory agencies as they determine what tissues should be monitored to ensure that the established residue safety tolerance levels are not exceeded.

UR - http://www.scopus.com/inward/record.url?scp=84940535357&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84940535357&partnerID=8YFLogxK

U2 - 10.5740/jaoacint.13-327

DO - 10.5740/jaoacint.13-327

M3 - Article

C2 - 26025372

AN - SCOPUS:84940535357

VL - 98

SP - 575

EP - 579

JO - Journal of AOAC International

JF - Journal of AOAC International

SN - 1060-3271

IS - 3

ER -