Ripple structure-generated hybrid electrokinetics for on-chip mixing and separating of functionalized beads

I. Fang Cheng, Sheng Chuan Chiang, Cheng Che Chung, Trai Ming Yeh, Hsien Chang Chang

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

We present an electrokinetics-based microfluidic platform that is capable of on-chip manipulating, mixing, and separating microparticles through adjusting the interrelated magnitudes of dielectrophoresis and AC electroosmosis. Hybrid electrokinetic phenomenon is generated from an electric field-induced micro-ripple structure made of ultraviolet-curable glue. Size-dependent particle separation and selective removal over the ripple structure is demonstrated successfully. Varying the waveform from sine-wave to square-wave allows generating a fluid convection at specific positions to mix the antibody-functionalized beads and antigen. Potential application in the bead-based immunoassay was also demonstrated for immuno-reaction and subsequently separating the bead-bead aggregate and non-binding beads on-chip.

Original languageEnglish
Article number061102
JournalBiomicrofluidics
Volume8
Issue number6
DOIs
Publication statusPublished - 2014 Dec 30

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Materials Science(all)
  • Condensed Matter Physics
  • Fluid Flow and Transfer Processes
  • Colloid and Surface Chemistry

Fingerprint Dive into the research topics of 'Ripple structure-generated hybrid electrokinetics for on-chip mixing and separating of functionalized beads'. Together they form a unique fingerprint.

Cite this