Abstract
We present an electrokinetics-based microfluidic platform that is capable of on-chip manipulating, mixing, and separating microparticles through adjusting the interrelated magnitudes of dielectrophoresis and AC electroosmosis. Hybrid electrokinetic phenomenon is generated from an electric field-induced micro-ripple structure made of ultraviolet-curable glue. Size-dependent particle separation and selective removal over the ripple structure is demonstrated successfully. Varying the waveform from sine-wave to square-wave allows generating a fluid convection at specific positions to mix the antibody-functionalized beads and antigen. Potential application in the bead-based immunoassay was also demonstrated for immuno-reaction and subsequently separating the bead-bead aggregate and non-binding beads on-chip.
Original language | English |
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Article number | 061102 |
Journal | Biomicrofluidics |
Volume | 8 |
Issue number | 6 |
DOIs | |
Publication status | Published - 2014 Dec 30 |
All Science Journal Classification (ASJC) codes
- Biomedical Engineering
- General Materials Science
- Condensed Matter Physics
- Fluid Flow and Transfer Processes
- Colloid and Surface Chemistry