Role of IL-16 in delayed-type hypersensitivity reaction

Takayuki Yoshimoto, Chrong Reen Wang, Toshihiko Yoneto, Akio Matsuzawa, William W. Cruikshank, Hideo Nariuchi

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54 Citations (Scopus)


Interleukin (IL)-16 is a chemoattractant cytokine for CD4+ leukocytes. Because delayed-type hypersensitivity (DTH) reaction is mediated by T helper 1 (Th1) cells and CD4+ T cells can be chemoattracted by IL-16, we have investigated the involvement of IL-16 in the DTH reaction. Immunohistochemical analysis revealed the IL-16 expression in infiltrating cells and epithelial cells in the DTH footpads. The IL-16 expression was also detected intracellularly in the Infiltrating cells. In addition, markedly increased production of IL-16 was detected in the DTH footpad extracts, but not in the control footpad extracts, by an enzyme-linked immunosorbent assay and also by Western blot analysis. The DTH footpad extracts exhibited a strong chemoattractant activity toward splenic T cells, which was significantly inhibited by the inclusion of neutralizing monoclonal antibody (mAb) against IL-16 in the migration assay. Furthermore, treatment of sensitized mice in vivo with the anti-lL-16 neutralizing mAb significantly suppressed the footpad swelling induced by an antigen challenge, together with decreased infiltration of leukocytes including not only CD4+ T cells but also CD8+ T cells and macrophages into the DTH footpads. Decreased production of macrophage inflammatory protein 1α was also observed in the DTH footpad extracts by the mAb treatment. These results suggest that IL-16 plays an important role in the recruitment of leukocytes-presumably including antigen-specific Th1 cells, which secrete cytokines and chemokines mediating the following hypersensitivity reaction after activation by the interaction with Langerhans cells carrying the antigen-for the elicitation of DTH response. (C) 2000 by The American Society of Hematology.

Original languageEnglish
Pages (from-to)2869-2874
Number of pages6
Issue number9
Publication statusPublished - 2000 May 1

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology


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