TY - JOUR
T1 - Role of Penaeus monodon hemocyte homeostasis associated protein (PmHHAP) in regulation of caspase-mediated apoptosis
AU - Apitanyasai, Kantamas
AU - Amparyup, Piti
AU - Charoensapsri, Walaiporn
AU - Senapin, Saengchan
AU - Tassanakajon, Anchalee
N1 - Funding Information:
This work was supported by research grants from the Thailand Research Fund (TRF Senior Scholar No. RTA5580008 ; IRG5780008 ) and the Ratchadaphiseksomphot Endowment Fund of Chulalongkorn University ( RES560530202-FW ). We also thanks the Japan Science and Technology Agency/Japan International Cooperation Agency, Science and Technology Research Partnership for Sustainable Development (JST/JICA, SATREPS) for equipment support. K.A. is the recipient of a student fellowship from the Royal Golden Jubilee Ph.D. Program, Joint Funding of the Thailand Research Fund and Chulalongkorn University and the 90th Anniversary of Chulalongkorn University Fund (Ratchadaphiseksomphot Endowment Fund). We acknowledge Chulalongkorn University for the support to the Center of Excellence for Molecular Biology and Genomics of Shrimp. We thank Dr. Robert Douglas John Butcher at the Publication Counseling Unit, Faculty of Science, Chulalongkorn University, for English language corrections of this manuscript.
Publisher Copyright:
© 2015 Elsevier Ltd.
PY - 2015/11/1
Y1 - 2015/11/1
N2 - The viral responsive protein, PmHHAP, plays an important role in the control of hemocyte homeostasis in shrimps during viral infection. In this study, we further investigate the role of PmHHAP in the regulation of hemocyte apoptosis. RNA interference (RNAi) mediated gene silencing was used to suppress the PmHHAP expression and the change in hemocyte apoptosis was determined in the knockdown shrimp. Within circulating hemocytes, PmHHAP knockdown increased the number of annexin V-positive apoptotic cells and the combined caspase-3/-7 activity and induced the characteristic apoptotic DNA ladder. Furthermore, PmHHAP down-regulation was accompanied by significantly altered expression of apoptosis-related proteins including the effector caspases, PmCaspase and PmCasp. Yeast two-hybrid and co-immunoprecipitation assays showed that PmHHAP binds to the p20 domain of PmCasp. Moreover, the recombinant PmHHAP protein was able to reduce the caspase activity in the actinomycin D-treated hemocyte cells and r. PmCasp-treated hemocyte cells. Taken together, our data indicate that PmHHAP regulates hemocyte homeostasis by inhibits apoptotic cell death through caspase activation.
AB - The viral responsive protein, PmHHAP, plays an important role in the control of hemocyte homeostasis in shrimps during viral infection. In this study, we further investigate the role of PmHHAP in the regulation of hemocyte apoptosis. RNA interference (RNAi) mediated gene silencing was used to suppress the PmHHAP expression and the change in hemocyte apoptosis was determined in the knockdown shrimp. Within circulating hemocytes, PmHHAP knockdown increased the number of annexin V-positive apoptotic cells and the combined caspase-3/-7 activity and induced the characteristic apoptotic DNA ladder. Furthermore, PmHHAP down-regulation was accompanied by significantly altered expression of apoptosis-related proteins including the effector caspases, PmCaspase and PmCasp. Yeast two-hybrid and co-immunoprecipitation assays showed that PmHHAP binds to the p20 domain of PmCasp. Moreover, the recombinant PmHHAP protein was able to reduce the caspase activity in the actinomycin D-treated hemocyte cells and r. PmCasp-treated hemocyte cells. Taken together, our data indicate that PmHHAP regulates hemocyte homeostasis by inhibits apoptotic cell death through caspase activation.
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U2 - 10.1016/j.dci.2015.06.004
DO - 10.1016/j.dci.2015.06.004
M3 - Article
C2 - 26111999
AN - SCOPUS:84938070953
SN - 0145-305X
VL - 53
SP - 234
EP - 243
JO - Developmental and Comparative Immunology
JF - Developmental and Comparative Immunology
IS - 1
ER -