Salinity-dependent expression of the branchial Na +/K +/2Cl - cotransporter and Na +/K +-ATPase in the sailfin molly correlates with hypoosmoregulatory endurance

Wen Kai Yang, Chao-Kai Kang, Tzu Ying Chen, Wen Been Chang, Tsung Han Lee

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

In the branchial mitochondrion-rich (MR) cells of euryhaline teleosts, the Na +/K +/2Cl - cotransporter (NKCC) is an important membrane protein that maintains the internal Cl - concentration, and the branchial Na +/K +-ATPase (NKA) is crucial for providing the driving force for many other ion-transporting systems. Hence this study used the sailfin molly (Poecilia latipinna), an introduced aquarium fish in Taiwan, to reveal that the potential roles of NKCC and NKA in sailfin molly were correlated to fish survival rates upon salinity challenge. Higher levels of branchial NKCC were found in seawater (SW)-acclimated sailfin molly compared to freshwater (FW)-acclimated individuals. Transfer of the sailfin molly from SW to FW revealed that the expression of the NKCC and NKA proteins in the gills was retained over 7 days in order to maintain hypoosmoregulatory endurance. Meanwhile, their survival rates after transfer to SW varied with the duration of FW-exposure and decreased significantly when the SW-acclimated individuals were acclimated to FW for 21 days. Double immunofluorescence staining showed that in SW-acclimated sailfin molly, NKCC signals were expressed on the basolateral membrane of MR cells, whereas in FW-acclimated molly, they were expressed on the apical membrane. This study illustrated the correlation between the gradual reductions in expression of branchial NKCC and NKA (i. e., the hypoosmoregulatory endurance) and decreasing survival rates after hyperosmotic challenge in sailfin molly.

Original languageEnglish
Pages (from-to)953-964
Number of pages12
JournalJournal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology
Volume181
Issue number7
DOIs
Publication statusPublished - 2011 Oct 1

Fingerprint

Poecilia
sodium-potassium-exchanging ATPase
Salinity
Seawater
Adenosine Triphosphatases
Durability
salinity
seawater
Fresh Water
Mitochondria
mitochondrion
membrane
Fish
survival rate
Membranes
protein
mitochondria
fish
aquarium
Fishes

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Biochemistry
  • Physiology
  • Animal Science and Zoology
  • Endocrinology

Cite this

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title = "Salinity-dependent expression of the branchial Na +/K +/2Cl - cotransporter and Na +/K +-ATPase in the sailfin molly correlates with hypoosmoregulatory endurance",
abstract = "In the branchial mitochondrion-rich (MR) cells of euryhaline teleosts, the Na +/K +/2Cl - cotransporter (NKCC) is an important membrane protein that maintains the internal Cl - concentration, and the branchial Na +/K +-ATPase (NKA) is crucial for providing the driving force for many other ion-transporting systems. Hence this study used the sailfin molly (Poecilia latipinna), an introduced aquarium fish in Taiwan, to reveal that the potential roles of NKCC and NKA in sailfin molly were correlated to fish survival rates upon salinity challenge. Higher levels of branchial NKCC were found in seawater (SW)-acclimated sailfin molly compared to freshwater (FW)-acclimated individuals. Transfer of the sailfin molly from SW to FW revealed that the expression of the NKCC and NKA proteins in the gills was retained over 7 days in order to maintain hypoosmoregulatory endurance. Meanwhile, their survival rates after transfer to SW varied with the duration of FW-exposure and decreased significantly when the SW-acclimated individuals were acclimated to FW for 21 days. Double immunofluorescence staining showed that in SW-acclimated sailfin molly, NKCC signals were expressed on the basolateral membrane of MR cells, whereas in FW-acclimated molly, they were expressed on the apical membrane. This study illustrated the correlation between the gradual reductions in expression of branchial NKCC and NKA (i. e., the hypoosmoregulatory endurance) and decreasing survival rates after hyperosmotic challenge in sailfin molly.",
author = "Yang, {Wen Kai} and Chao-Kai Kang and Chen, {Tzu Ying} and Chang, {Wen Been} and Lee, {Tsung Han}",
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T1 - Salinity-dependent expression of the branchial Na +/K +/2Cl - cotransporter and Na +/K +-ATPase in the sailfin molly correlates with hypoosmoregulatory endurance

AU - Yang, Wen Kai

AU - Kang, Chao-Kai

AU - Chen, Tzu Ying

AU - Chang, Wen Been

AU - Lee, Tsung Han

PY - 2011/10/1

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N2 - In the branchial mitochondrion-rich (MR) cells of euryhaline teleosts, the Na +/K +/2Cl - cotransporter (NKCC) is an important membrane protein that maintains the internal Cl - concentration, and the branchial Na +/K +-ATPase (NKA) is crucial for providing the driving force for many other ion-transporting systems. Hence this study used the sailfin molly (Poecilia latipinna), an introduced aquarium fish in Taiwan, to reveal that the potential roles of NKCC and NKA in sailfin molly were correlated to fish survival rates upon salinity challenge. Higher levels of branchial NKCC were found in seawater (SW)-acclimated sailfin molly compared to freshwater (FW)-acclimated individuals. Transfer of the sailfin molly from SW to FW revealed that the expression of the NKCC and NKA proteins in the gills was retained over 7 days in order to maintain hypoosmoregulatory endurance. Meanwhile, their survival rates after transfer to SW varied with the duration of FW-exposure and decreased significantly when the SW-acclimated individuals were acclimated to FW for 21 days. Double immunofluorescence staining showed that in SW-acclimated sailfin molly, NKCC signals were expressed on the basolateral membrane of MR cells, whereas in FW-acclimated molly, they were expressed on the apical membrane. This study illustrated the correlation between the gradual reductions in expression of branchial NKCC and NKA (i. e., the hypoosmoregulatory endurance) and decreasing survival rates after hyperosmotic challenge in sailfin molly.

AB - In the branchial mitochondrion-rich (MR) cells of euryhaline teleosts, the Na +/K +/2Cl - cotransporter (NKCC) is an important membrane protein that maintains the internal Cl - concentration, and the branchial Na +/K +-ATPase (NKA) is crucial for providing the driving force for many other ion-transporting systems. Hence this study used the sailfin molly (Poecilia latipinna), an introduced aquarium fish in Taiwan, to reveal that the potential roles of NKCC and NKA in sailfin molly were correlated to fish survival rates upon salinity challenge. Higher levels of branchial NKCC were found in seawater (SW)-acclimated sailfin molly compared to freshwater (FW)-acclimated individuals. Transfer of the sailfin molly from SW to FW revealed that the expression of the NKCC and NKA proteins in the gills was retained over 7 days in order to maintain hypoosmoregulatory endurance. Meanwhile, their survival rates after transfer to SW varied with the duration of FW-exposure and decreased significantly when the SW-acclimated individuals were acclimated to FW for 21 days. Double immunofluorescence staining showed that in SW-acclimated sailfin molly, NKCC signals were expressed on the basolateral membrane of MR cells, whereas in FW-acclimated molly, they were expressed on the apical membrane. This study illustrated the correlation between the gradual reductions in expression of branchial NKCC and NKA (i. e., the hypoosmoregulatory endurance) and decreasing survival rates after hyperosmotic challenge in sailfin molly.

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