Salvianolic acid B modulates hemostasis properties of human umbilical vein endothelial cells

Chung Sheng Shi, Huey Chun Huang, Hua Lin Wu, Cheng Hsiang Kuo, Bi Ing Chang, Ming shi Shiao, Guey Yueh Shi

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)

Abstract

Salviae miltiorrhizae (SM), a clinical, commonly used herb, can activate blood circulation and resolve stasis. We have investigated the effects of salvianolic acid B (Sal B), a pure compound extracted from the dried SM roots, on fibrinolytic (tissue-type plasminogen activator and plasminogen activator inhibitor, t-PA and PAI) and anticoagulant (thrombomodulin,TM) properties of cultured human umbilical vein endothelial cells (HUVECs). When HUVECs were treated with Sal B, a dose- (0.0125-0.5 mg/ml) and a time-dependent decrease in PAI activity were observed. PAI type 1 (PAI-1) antigen and PAI-1 mRNA expression significantly decreased compared to control values in the conditioned media of HUVECs pretreated with Sal B for 12 h. Moreover, TM activity reached a maximum stimulation of 1.25-fold over control levels in the pretreatment of Sal B for 12 h and t-PA and TM specific mRNA expression also increased (1.7- and 1.8-fold, respectively). In conclusion, Sal B increased the fibrinolytic and anticoagulant potential of cultured HUVECs by up-regulating the expression of t-PA and TM and by down-regulating the expression of PAI-1. These data suggest that Sal B is clinically effective because of its ability to change the gene expression profile of endothelial cells thereby preventing vascular events.

Original languageEnglish
Pages (from-to)769-775
Number of pages7
JournalThrombosis Research
Volume119
Issue number6
DOIs
Publication statusPublished - 2007 Mar 23

All Science Journal Classification (ASJC) codes

  • Hematology

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